3' RNA ligase mediated rapid amplification of cDNA ends for validating viroid induced cleavage at the 3' extremity of the host mRNA.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2017.09.023
Abstract: 5' RNA ligase-mediated rapid amplification of cDNA ends (5' RLM-RACE) is a widely-accepted method for the validation of direct cleavage of a target gene by a microRNA (miRNA) and viroid-derived small RNA (vd-sRNA). However, this… read more here.
Keywords: cleavage; mediated rapid; rna ligase; ligase mediated ... See more keywords
High-throughput sequencing reveals circular substrates for an archaeal RNA ligase
Sign Up to like & getrecommendations! Published in 2017 at "RNA Biology"
DOI: 10.1080/15476286.2017.1302640
Abstract: ABSTRACT It is only recently that the abundant presence of circular RNAs (circRNAs) in all kingdoms of Life, including the hyperthermophilic archaeon Pyrococcus abyssi, has emerged. This led us to investigate the physiologic significance of… read more here.
Keywords: rna ligase; reveals circular; sequencing reveals; throughput sequencing ... See more keywords
Direct adenylation from 5′-OH-terminated oligonucleotides by a fusion enzyme containing Pfu RNA ligase and T4 polynucleotide kinase
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac604
Abstract: Abstract 5′-Adenylated oligonucleotides (AppOligos) are widely used for single-stranded DNA/RNA ligation in next-generation sequencing (NGS) applications such as microRNA (miRNA) profiling. The ligation between an AppOligo adapter and target molecules (such as miRNA) no longer… read more here.
Keywords: rna ligase; pfu rna; rna; fusion ... See more keywords
Enzymatic Conjugation of Modified RNA Fragments by Ancestral RNA Ligase AncT4_2
Sign Up to like & getrecommendations! Published in 2022 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.01679-22
Abstract: RNA therapeutics is one of the next-generation medicines for treating various diseases. Our designed ancestral RNA ligase AncT4_2 exhibited excellent enzymatic properties, such as high thermal stability, productivity, specific activity, and broad substrate selectivity compared… read more here.
Keywords: modified rna; rna; anct4; rna fragments ... See more keywords