Genetically Encoded, Functional Single-Strand RNA Origami: Anticoagulant.
Sign Up to like & getrecommendations! Published in 2019 at "Advanced materials"
DOI: 10.1002/adma.201808262
Abstract: Nucleic acid aptamers selected for thrombin binding have been previously shown to possess anticoagulant activity; however, problems with rapid renal clearance and short circulation half-life have prevented translation to clinical usefulness. Here, a family of… read more here.
Keywords: rna origami; anticoagulant activity; rna; dna ... See more keywords
Synthetic Translational Regulation by Protein-Binding RNA Origami Scaffolds
Sign Up to like & getrecommendations! Published in 2022 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.1c00608
Abstract: Rational design approaches for the regulation of gene expression are expanding the synthetic biology toolbox. However, only a few tools for regulating gene expression at the translational level have been developed. Here, we devise an… read more here.
Keywords: translational regulation; rna origami; origami scaffolds; regulation ... See more keywords
Utilizing RNA origami scaffolds in Saccharomyces cerevisiae for dCas9-mediated transcriptional control
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DOI: 10.1093/nar/gkac470
Abstract: Abstract Designer RNA scaffolds constitute a promising tool for synthetic biology, as they can be genetically expressed to perform specific functions in vivo such as scaffolding enzymatic cascades and regulating gene expression through CRISPR-dCas9 applications.… read more here.
Keywords: control; rna; scaffolds saccharomyces; rna origami ... See more keywords
RNA origami scaffolds facilitate cryo-EM characterization of a Broccoli–Pepper aptamer FRET pair
Sign Up to like & getrecommendations! Published in 2023 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkad224
Abstract: Abstract Cryogenic electron microscopy (cryo-EM) is a promising method for characterizing the structure of larger RNA structures and complexes. However, the structure of individual aptamers is difficult to solve by cryo-EM due to their low… read more here.
Keywords: structure; cryo; rna origami; origami scaffolds ... See more keywords