Domain structure of HelD, an interaction partner of Bacillus subtilis RNA polymerase
Sign Up to like & getrecommendations! Published in 2019 at "FEBS Letters"
DOI: 10.1002/1873-3468.13385
Abstract: The HelD is a helicase‐like protein binding to Bacillus subtilis RNA polymerase (RNAP), stimulating transcription in an ATP‐dependent manner. Here, our small angle X‐ray scattering data bring the first insights into the HelD structure: HelD… read more here.
Keywords: domain structure; rna polymerase; structure held; structure ... See more keywords
RNA polymerase efficiently transcribes through DNA‐scaffolded, cooperative bacteriophage repressor complexes
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Letters"
DOI: 10.1002/1873-3468.14447
Abstract: DNA can act as a scaffold for the cooperative binding of protein oligomers. For example, the phage 186 CI repressor forms a wheel of seven dimers wrapped in DNA with specific binding sites, while phage… read more here.
Keywords: dna; repressor; transcription; rna polymerase ... See more keywords
Effective 5‐aminolevulinic acid production via T7 RNA polymerase and RuBisCO equipped Escherichia coli W3110
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28273
Abstract: Chromosome‐based engineering is a superior approach for gene integration generating a stable and robust chassis. Therefore, an effective amplifier, T7 RNA polymerase (T7RNAP) from bacteriophage, has been incorporated into Escherichia coli W3110 by site‐specific integration.… read more here.
Keywords: aminolevulinic acid; escherichia coli; rna polymerase; production ... See more keywords
A computational comparative analysis of the binding mechanism of molnupiravir's active metabolite to RNA‐dependent RNA polymerase of wild‐type and Delta subvariant AY.4 of SARS‐CoV‐2
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Cellular Biochemistry"
DOI: 10.1002/jcb.30226
Abstract: The antiviral drug molnupiravir targets the SARS‐CoV‐2 RNA‐dependent RNA polymerase (RdRP) enzyme. Early treatment with molnupiravir reduced the risk of hospitalization or death in at‐risk, unvaccinated adults with COVID‐19, according to phase 3 clinical trials.… read more here.
Keywords: dependent rna; rna polymerase; rdrp; rna ... See more keywords
Drug repurposing toward the inhibition of RNA‐dependent RNA polymerase of various flaviviruses through computational study
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Cellular Biochemistry"
DOI: 10.1002/jcb.30352
Abstract: Numerous pathogens affecting human is present in the flavivirus family namely west nile, dengue, yellow fever, and zika which involves in development of global burden and distressing the environment economically. Till date, no approved drugs… read more here.
Keywords: rna dependent; dependent rna; rna polymerase; inhibition ... See more keywords
Drug repurposing for Mpox: Discovery of small molecules as potential inhibitors against DNA‐dependent RNA polymerase using molecular modeling approach
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Cellular Biochemistry"
DOI: 10.1002/jcb.30397
Abstract: Mpox (formerly Monkeypox), a zoonotic illness caused by the Mpox virus, belongs to the Orthopoxvirus genus in the family Poxviridae. To design and develop effective antiviral therapeutics against DNA viruses, the DNA‐dependent RNA polymerase (DdRp)… read more here.
Keywords: dependent rna; rna polymerase; kcal mol; dna dependent ... See more keywords
Corrigendum to: The potential chemical structure of anti-SARS-CoV-2 RNA-dependent RNA polymerase (Journal of Medical Virology, (2020), 92, 6, (693-697), 10.1002/jmv.25761)
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Medical Virology"
DOI: 10.1002/jmv.26176
Abstract: The potential chemical structure of anti-SARS-CoV-2 RNA-dependent RNA polymerase. The article to which this Corrigendum refers was published in Journal of Medical Virology 92(6): 693 to 697 (https://onlinelibrary.wiley.com/doi/full/10.1002/jmv.25761). The corrigendum corrects protease to polymerase in… read more here.
Keywords: dependent rna; rna dependent; rna polymerase; rna ... See more keywords
Studying transcription initiation by RNA polymerase with diffusion‐based single‐molecule fluorescence
Sign Up to like & getrecommendations! Published in 2017 at "Protein Science"
DOI: 10.1002/pro.3160
Abstract: Over the past decade, fluorescence‐based single‐molecule studies significantly contributed to characterizing the mechanism of RNA polymerase at different steps in transcription, especially in transcription initiation. Transcription by bacterial DNA‐dependent RNA polymerase is a multistep process… read more here.
Keywords: transcription; transcription initiation; single molecule; rna polymerase ... See more keywords
Observing Single RNA Polymerase Molecules Down to Base-Pair Resolution.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6421-5_15
Abstract: During transcriptional elongation, RNA polymerases (RNAP) employ a stepping mechanism to translocate along the DNA template while synthesizing RNA. Optical trapping assays permit the progress of single molecules of RNA polymerase to be monitored in… read more here.
Keywords: transcriptional elongation; rna polymerase; elongation; optical trapping ... See more keywords
Detection of RNA Polymerase II in Mouse Embryos During Zygotic Genome Activation Using Immunocytochemistry.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6988-3_10
Abstract: Mammalian pre-implantation embryos represent a highly dynamic experimental model for comparative studies of nuclear structure and functions in the context of gradual reactivation of transcription. Here, we present details of the methods that allow localizing… read more here.
Keywords: detection rna; rna polymerase; polymerase mouse;
Discovery of Potent Non-nucleoside Inhibitors of Dengue Viral RNA-Dependent RNA Polymerase from Fragment Screening and Structure-Guided Design.
Sign Up to like & getrecommendations! Published in 2018 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-981-10-8727-1_14
Abstract: Flavivirus NS5 RNA-dependent RNA polymerase (RdRp) is an important drug target. Whilst a number of allosteric inhibitors have been described for Hepatitis C virus RdRp, few have been described for DENV RdRp. In addition, compound… read more here.
Keywords: rna dependent; rna; structure guided; rna polymerase ... See more keywords