Synthesis of Single-Stranded RNA Probes by In Vitro Transcription.
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DOI: 10.1101/pdb.prot100628
Abstract: In this protocol, we describe procedures for synthesizing RNA probes of high specific activity from DNA templates containing promoters for bacteriophage-encoded RNA polymerases. The DNA fragment to be transcribed should have previously been cloned into… read more here.
Keywords: single stranded; vitro transcription; synthesis single; rna probes ... See more keywords