Engineer RNA-Protein Nanowires as Light-Responsive Biomaterials.
Sign Up to like & getrecommendations! Published in 2023 at "Small"
DOI: 10.1002/smll.202206513
Abstract: RNA molecules have emerged as increasingly attractive biomaterials with important applications such as RNA interference (RNAi) for cancer treatment and mRNA vaccines against infectious diseases. However, it remains challenging to engineer RNA biomaterials with sophisticated… read more here.
Keywords: light responsive; engineer rna; protein nanowires; rna protein ... See more keywords
Preparation of SNAPf-Beads for Colocalization Single-Molecule Spectroscopy (CoSMoS) of RNA-Protein Complexes.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0278-2_2
Abstract: The SNAPf-tag is a chemical tag that allows rapid and highly specific covalent labeling of proteins even in the full complexity of the cellular environment. The SNAPf-tag has been instrumental to study native RNA-protein complexes… read more here.
Keywords: snapf; single molecule; protein complexes; spectroscopy ... See more keywords
Plant Individual Nucleotide Resolution Cross-Linking and Immunoprecipitation to Characterize RNA-Protein Complexes.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0712-1_15
Abstract: In recent years, it has become increasingly recognized that regulation at the RNA level pervasively shapes the transcriptome in eukaryotic cells. This has fostered an interest in the mode of action of RNA-binding proteins that,… read more here.
Keywords: protein; linking immunoprecipitation; cross linking; rna protein ... See more keywords
Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein: Analyses of miRNA and Protein Expression.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1433-4_1
Abstract: Several studies have indicated the presence of microRNAs (miRNAs) within mitochondria although the origin, as well as the biological function, of these mitochondrially located miRNAs is largely unknown. The identification and significance of this subcellular… read more here.
Keywords: protein; rna; total rna; extraction total ... See more keywords
Simultaneous DNA, RNA, and Protein Analysis from Single Cells Using a High-Throughput Microfluidic Workflow for Resolution of Genotype-to-Phenotype Modalities.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1771-7_17
Abstract: Understanding the genomic landscape of cancer in single cells can be valuable for the characterization of molecular events that drive evolution of tumorigenesis and fostering progress in identifying druggable regimens for patient treatment scenarios. We… read more here.
Keywords: cells using; dna rna; single cells; simultaneous dna ... See more keywords
Finding RNA-Protein Interaction Sites Using HMMs.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6753-7_13
Abstract: RNA-binding proteins play important roles in the various stages of RNA maturation through binding to its target RNAs. Cross-linking immunoprecipitation coupled with high-throughput sequencing (CLIP-Seq) has made it possible to identify the targeting sites of… read more here.
Keywords: protein; clip seq; protein interaction; rna binding ... See more keywords
Trimolecular Fluorescence Complementation (TriFC) Assay for Visualization of RNA-Protein Interaction in Plants.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9045-0_19
Abstract: RNA-protein interactions play important roles in various eukaryotic biological processes. Molecular imaging of subcellular localization of RNA-protein complexes in plants is critical for understanding these interactions. However, methods to image RNA-protein interactions in living plants… read more here.
Keywords: protein interaction; fluorescence complementation; rna; rna protein ... See more keywords
Methods for Identification of Protein-RNA Interaction.
Sign Up to like & getrecommendations! Published in 2018 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-981-13-0719-5_12
Abstract: The importance of RNA-protein interactions in regulation of mRNA and non-coding RNA function is increasingly appreciated. With the development of next generation high-throughput sequencing technologies, a variety of methods have been proposed to comprehensively identify RNA-protein… read more here.
Keywords: protein interaction; rna protein; protein interactions; interaction ... See more keywords
The Central Symbiosis of Molecular Biology: Molecules in Mutualism
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Molecular Evolution"
DOI: 10.1007/s00239-017-9804-x
Abstract: As illustrated by the mitochondrion and the eukaryotic cell, little in biology makes sense except in light of mutualism. Mutualisms are persistent, intimate, and reciprocal exchanges; an organism proficient in obtaining certain benefits confers those… read more here.
Keywords: central symbiosis; biology; mutualism; molecules mutualism ... See more keywords
Cytoplasmic RNA-Protein Particles Exhibit Non-Gaussian Subdiffusive Behavior.
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical journal"
DOI: 10.1016/j.bpj.2016.11.3208
Abstract: The cellular cytoplasm is a complex, heterogeneous environment (both spatially and temporally) that exhibits viscoelastic behavior. To further develop our quantitative insight into cellular transport, we analyze data sets of mRNA molecules fluorescently labeled with… read more here.
Keywords: non gaussian; protein particles; particles exhibit; behavior ... See more keywords
Prediction of RNA-protein interactions by combining deep convolutional neural network with feature selection ensemble method.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of theoretical biology"
DOI: 10.1016/j.jtbi.2018.10.029
Abstract: RNA-protein interaction (RPI) plays an important role in the basic cellular processes of organisms. Unfortunately, due to time and cost constraints, it is difficult for biological experiments to determine the relationship between RNA and protein… read more here.
Keywords: protein; network; feature; method ... See more keywords