Monitoring Enzymatic RNA G-Quadruplex Unwinding Activities by Nuclease Sensitivity and Reverse Transcription Stop Assays.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0935-4_11
Abstract: Multiple different methods have been employed to investigate the unwinding of RNA G-quadruplexes by various helicase proteins. Each has their own pitfalls, namely, looking at non-native or chemically modified RNA sequences, biasing the unwinding process… read more here.
Keywords: quadruplex unwinding; enzymatic rna; rna; monitoring enzymatic ... See more keywords
Stabilization of human telomeric RNA G-quadruplex by the water-compatible optically pure and biologically-active metallohelices
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-71429-5
Abstract: RNA G-quadruplexes have been suggested to play key roles in fundamental biological processes and are linked to human diseases. Thus, they also represent good potential therapeutic targets. Here, we describe, using the methods of molecular… read more here.
Keywords: human telomeric; stabilization; rna; telomeric rna ... See more keywords
Ligand-based drug repurposing strategy identified SARS-CoV-2 RNA G-quadruplex binders.
Sign Up to like & getrecommendations! Published in 2022 at "Chemical communications"
DOI: 10.1039/d2cc03135c
Abstract: The single-stranded RNA genome of SARS-CoV-2 contains some G-quadruplex-forming G-rich elements which are putative drug targets. Here, we performed a ligand-based pharmacophore virtual screening of FDA approved drugs to find candidates targeting such RNA structures.… read more here.
Keywords: rna quadruplex; quadruplex binders; ligand based; cov rna ... See more keywords
Small molecule-based detection of non-canonical RNA G-quadruplex structures that modulate protein translation
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac580
Abstract: Abstract Tandem repeats of guanine-rich sequences in RNA often form thermodynamically stable four-stranded RNA structures. Such RNA G-quadruplexes have long been considered to be linked to essential biological processes, yet their physiological significance in cells… read more here.
Keywords: rna quadruplex; protein translation; rna; modulate protein ... See more keywords
QUADRatlas: the RNA G-quadruplex and RG4-binding proteins database
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac782
Abstract: Abstract RNA G-quadruplexes (RG4s) are non-canonical, disease-associated post-transcriptional regulators of gene expression whose functions are driven by RNA-binding proteins (RBPs). Being able to explore transcriptome-wide RG4 formation and interaction with RBPs is thus paramount to… read more here.
Keywords: rg4; quadruplex rg4; rna quadruplex; rg4s ... See more keywords
The different activities of RNA G-quadruplex structures are controlled by flanking sequences.
Sign Up to like & getrecommendations! Published in 2022 at "Life science alliance"
DOI: 10.26508/lsa.202101232
Abstract: The role of G-quadruplex (G4) RNA structures is multifaceted and controversial. Here, we have used as a model the EBV-encoded EBNA1 and the Kaposi's sarcoma-associated herpesvirus (KSHV)-encoded LANA1 mRNAs. We have compared the G4s in… read more here.
Keywords: activities rna; structures controlled; different activities; quadruplex structures ... See more keywords
RNA G-quadruplex forming regions from SARS-2, SARS-1 and MERS coronoviruses
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Chemistry"
DOI: 10.3389/fchem.2022.1014663
Abstract: COVID-19 (Corona Virus Disease 2019), SARS (Severe Acute Respiratory Syndrome) and MERS (Middle East Respiratory Syndrome) are infectious diseases each caused by coronavirus outbreaks. Small molecules and other therapeutics are rapidly being developed to treat… read more here.
Keywords: forming regions; regions sars; rna quadruplex; quadruplex forming ... See more keywords
RNA G-Quadruplex within the 5′-UTR of FEN1 Regulates mRNA Stability under Oxidative Stress
Sign Up to like & getrecommendations! Published in 2023 at "Antioxidants"
DOI: 10.3390/antiox12020276
Abstract: Reactive oxygen species (ROS) are a group of highly oxidative molecules that induce DNA damage, affecting DNA damage response (DDR) and gene expression. It is now recognized that DNA base excision repair (BER) is one… read more here.
Keywords: utr fen1; oxidative stress; fen1; rna quadruplex ... See more keywords
Naphthalene Diimides Carrying Two β-Cyclodextrins Prefer Telomere RNA G-Quadruplex Recognition
Sign Up to like & getrecommendations! Published in 2022 at "Molecules"
DOI: 10.3390/molecules27134053
Abstract: Newly synthesized naphthalene diimide carrying two β-cyclodextrins (NDI-β-CyDs) showed improved specificity for the parallel G-quadruplex structure alongside the hybrid G-quadruplex structure. Specifically, the highest binding affinity of NDI-β-CyDs for the telomere RNA G-quadruplex was observed.… read more here.
Keywords: two cyclodextrins; telomere rna; naphthalene diimides; rna quadruplex ... See more keywords
In vivo action of RNA G-quadruplex in phloem development
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DOI: 10.5483/bmbrep.2018.51.11.253
Abstract: Phloem network integrates cellular energy status into post-embryonic growth, and development by tight regulation of carbon allocation. Phloem development involves complicated coordination of cell fate determination, cell division, and terminal differentiation into sieve elements (SEs),… read more here.
Keywords: phloem; vivo action; rna quadruplex; phloem development ... See more keywords