Detecting RNA–RNA interactome
Sign Up to like & getrecommendations! Published in 2022 at "Wiley Interdisciplinary Reviews: RNA"
DOI: 10.1002/wrna.1715
Abstract: The last decade has seen a robust increase in various types of novel RNA molecules and their complexity in gene regulation. RNA molecules play a critical role in cellular events by interacting with other biomolecules,… read more here.
Keywords: rna interactome; rna molecules; rna; detecting rna ... See more keywords
Single-Molecule FRET Assay to Observe the Activity of Proteins Involved in RNA/RNA Annealing.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7634-8_17
Abstract: In recent years, single-molecule fluorescence resonance energy transfer (smFRET) has emerged as a powerful technique to study macromolecular interactions. The chief advantages of smFRET analysis compared to bulk measurements include the possibility to detect sample… read more here.
Keywords: methodology; single molecule; rna; molecule fret ... See more keywords
The virulence of NDV NA-1 strain regulated by the 3' leader or 5' trailer sequences.
Sign Up to like & getrecommendations! Published in 2019 at "Microbial pathogenesis"
DOI: 10.1016/j.micpath.2018.10.040
Abstract: The 3' and 5' terminal regions of Newcastle disease virus (NDV) genome are cis-acting regulatory elements involved in replication, transcription, and packaging of genomic and anti-genomic viral RNA. There are 6 different nucleotides (nts) at… read more here.
Keywords: strain; virulence; leader trailer; rna rna ... See more keywords
An In Vitro Assembly System Identifies Roles for RNA Nucleation and ATP in Yeast Stress Granule Formation.
Sign Up to like & getrecommendations! Published in 2020 at "Molecular cell"
DOI: 10.1016/j.molcel.2020.07.017
Abstract: Stress granules (SGs) are condensates of mRNPs that form in response to stress. SGs arise by multivalent protein-protein, protein-RNA, and RNA-RNA interactions. However, the role of RNA-RNA interactions in SG assembly remains understudied. Here, we… read more here.
Keywords: assembly system; formation; vitro assembly; stress ... See more keywords
An RNA–RNA crosstalk network involving HMGB1 and RICTOR facilitates hepatocellular carcinoma tumorigenesis by promoting glutamine metabolism and impedes immunotherapy by PD-L1+ exosomes activity
Sign Up to like & getrecommendations! Published in 2021 at "Signal Transduction and Targeted Therapy"
DOI: 10.1038/s41392-021-00801-2
Abstract: Hepatocellular carcinoma (HCC) is the global leading cause of cancer-related deaths due to the deficiency of targets for precision therapy. A new modality of epigenetic regulation has emerged involving RNA–RNA crosstalk networks where two or… read more here.
Keywords: rna rna; rictor; hmgb1; crosstalk ... See more keywords
RNA helicases regulate RNA condensates
Sign Up to like & getrecommendations! Published in 2020 at "Cell Research"
DOI: 10.1038/s41422-020-0296-7
Abstract: Stress granules are cytoplasmic membraneless RNA condensates for storing non-translating mRNAs, which are induced in response to various forms of stress. In a recent issue of Cell, Tauber et al. show that RNA helicases, particularly… read more here.
Keywords: rna rna; rna; rna interactions; formation ... See more keywords
An architectural role of specific RNA–RNA interactions in oskar granules
Sign Up to like & getrecommendations! Published in 2024 at "Nature Cell Biology"
DOI: 10.1038/s41556-024-01519-3
Abstract: Ribonucleoprotein (RNP) granules are membraneless condensates that organize the intracellular space by compartmentalization of specific RNAs and proteins. Studies have shown that RNA tunes the phase behaviour of RNA-binding proteins, but the role of intermolecular… read more here.
Keywords: rnp granules; role; specific rna; rna rna ... See more keywords
RIC-seq for global in situ profiling of RNA–RNA spatial interactions
Sign Up to like & getrecommendations! Published in 2020 at "Nature"
DOI: 10.1038/s41586-020-2249-1
Abstract: Highly structured RNA molecules usually interact with each other, and associate with various RNA-binding proteins, to regulate critical biological processes. However, RNA structures and interactions in intact cells remain largely unknown. Here, by coupling proximity… read more here.
Keywords: seq; rna binding; ric seq; seq global ... See more keywords
Global in situ profiling of RNA-RNA spatial interactions with RIC-seq.
Sign Up to like & getrecommendations! Published in 2021 at "Nature protocols"
DOI: 10.1038/s41596-021-00524-2
Abstract: Emerging evidence has demonstrated that RNA-RNA interactions are vital in controlling diverse biological processes, including transcription, RNA splicing and protein translation. RNA in situ conformation sequencing (RIC-seq) is a technique for capturing protein-mediated RNA-RNA proximal… read more here.
Keywords: rna rna; rna; rna interactions; ric seq ... See more keywords
Hfq-licensed RNA–RNA interactome in Pseudomonas aeruginosa reveals a keystone sRNA
Sign Up to like & getrecommendations! Published in 2023 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2218407120
Abstract: Significance Hfq is a key posttranscriptional regulator that modulates base-pairing interactions between sRNAs and their target mRNAs. Although there are more than 100 different sRNAs in Pseudomonas aeruginosa, information on their regulatory targets was available… read more here.
Keywords: rna interactome; hfq licensed; pseudomonas aeruginosa; interactome pseudomonas ... See more keywords
Identification of host RNAs that interact with EBV noncoding RNA EBER2
Sign Up to like & getrecommendations! Published in 2018 at "RNA Biology"
DOI: 10.1080/15476286.2018.1518854
Abstract: ABSTRACT Epstein-Barr virus (EBV) expresses an abundant nuclear noncoding RNA called EBER2, which interacts with and acts as a guide RNA for the host transcription factor PAX5. This ribonucleoprotein complex localizes to the terminal repeat… read more here.
Keywords: noncoding rna; rna; host; identification ... See more keywords