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   Articles with "rna situ" as a keyword



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Small RNA In Situ Hybridizations on Sections of Arabidopsis Embryos.

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recommendations! Published in 2020 at "Methods in molecular biology"

DOI: 10.1007/978-1-0716-0342-0_7

Abstract: Small RNAs mediate posttranscriptional gene silencing in plants and animals. This often occurs in specific cell or tissue types and can be necessary for their differentiation. Determining small RNA (sRNA) localization patterns at cellular resolution… read more here.

Keywords: small rna; hybridizations sections; sections arabidopsis; rna situ ... See more keywords

RNA In Situ Hybridization of Detecting Cucumber Mosaic Virus in Shoots of Nicotiana benthamiana Plants.

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recommendations! Published in 2022 at "Methods in molecular biology"

DOI: 10.1007/978-1-0716-1835-6_27

Abstract: RNA in situ hybridization, a histological technique derived from Southern blotting and northern blotting, has been an important approach in biology studies for many years. In the studies of virus-plant interactions, RNA in situ hybridization… read more here.

Keywords: cucumber mosaic; mosaic virus; rna situ; biology ... See more keywords

RNA In Situ Hybridization on Planarian Paraffin Sections.

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recommendations! Published in 2018 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-7802-1_13

Abstract: RNA in situ hybridization techniques are an important tool for the study of gene expression patterns in freshwater planarians. Here I describe a RNA in situ hybridization method on histological paraffin sections of planarian tissue.… read more here.

Keywords: hybridization planarian; rna situ; situ hybridization; paraffin sections ... See more keywords

Mapping human tissues with highly multiplexed RNA in situ hybridization

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recommendations! Published in 2024 at "Nature Communications"

DOI: 10.1038/s41467-024-46437-y

Abstract: In situ transcriptomic techniques promise a holistic view of tissue organization and cell-cell interactions. There has been a surge of multiplexed RNA in situ mapping techniques but their application to human tissues has been limited… read more here.

Keywords: human tissues; rna situ; cell; multiplexed rna ... See more keywords

RNA in situ hybridization and expression of related genes regulating the accumulation of triterpenoids in Cyclocarya paliurus.

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recommendations! Published in 2021 at "Tree physiology"

DOI: 10.1093/treephys/tpab067

Abstract: Cyclocarya paliurus, a woody medicinal species in the Juglandaceae, grows extensively in subtropical areas of China. Triterpenoids in the leaves have health-promoting effects, including hypoglycemic and hypolipidemic activities. To understand triterpenoid biosynthesis, transport, and accumulation… read more here.

Keywords: accumulation; expression; paliurus; gene ... See more keywords

RNA In Situ Hybridization of Whole-Mount Mosquito Olfactory Tissues.

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recommendations! Published in 2022 at "Cold Spring Harbor protocols"

DOI: 10.1101/pdb.prot107916

Abstract: This protocol presents a method for RNA in situ hybridization of whole-mounted preparations of mosquito antennae and maxillary palps. It can be adapted for other peripheral appendages such as the legs or proboscis. read more here.

Keywords: mount mosquito; situ hybridization; hybridization whole; rna situ ... See more keywords

The utility of ETV1, ETV4 and ETV5 RNA in‐situ hybridization in the diagnosis of CIC–DUX sarcomas

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recommendations! Published in 2017 at "Histopathology"

DOI: 10.1111/his.13112

Abstract: A recently characterized group of undifferentiated small round cell sarcomas harbours fusions of the genes CIC and DUX4. Studies report a distinctive gene expression profile for these sarcomas, including expression of E26 transformation‐specific (ETS) family… read more here.

Keywords: sarcomas; etv4 etv5; utility; etv1 etv4 ... See more keywords
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Evaluating the effectiveness of RNA in‐situ hybridization for detecting lung adenocarcinoma with anaplastic lymphoma kinase rearrangement

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recommendations! Published in 2017 at "Histopathology"

DOI: 10.1111/his.13198

Abstract: An easy and rapid assay for detecting mRNA in formalin‐fixed paraffin‐embedded samples [RNA in‐situ hybridization (ISH)] has been reported recently. The aim of this study was to investigate the diagnostic accuracy of RNA ISH in… read more here.

Keywords: lung adenocarcinoma; detecting lung; anaplastic lymphoma; rna situ ... See more keywords

Novel In Situ Hybridization Assay for Chromogenic Single-Molecule Detection of Human Papillomavirus E6/E7 mRNA

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recommendations! Published in 2023 at "Microbiology Spectrum"

DOI: 10.1128/spectrum.03896-22

Abstract: In situ detection of viral mRNA expression in tissue samples is of great value for pathological diagnosis to access viral infection status. Unfortunately, conventional RNA in situ hybridization assays lack sensitivity and specificity for clinical… read more here.

Keywords: situ hybridization; mrna; hybridization; hybridization assay ... See more keywords

Unmasking Budgerigar Splenic Leukocyte Populations With Single‐Cell Transcriptomics and Multiplex RNA In Situ Hybridization

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recommendations! Published in 2025 at "Journal of Immunology Research"

DOI: 10.1155/jimr/1554863

Abstract: The current understanding of the avian immune system primarily stems from research conducted in chickens, given their economic significance as a food source. Extending the research to other avian species like Psittaciformes requires the use… read more here.

Keywords: transcriptomics multiplex; single cell; rna situ; multiplex rna ... See more keywords

AR-V7 detection by a novel junction-specific RNA in situ hybridization (RISH) test.

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recommendations! Published in 2017 at "Journal of Clinical Oncology"

DOI: 10.1200/jco.2017.35.6_suppl.191

Abstract: 191Background: Previous studies have reported an association between blood-based detection of androgen receptor splice variant-7 (AR-V7) and resistance to abiraterone/enzalutamide in men with metastatic castration-resistant prostate cancer (mCRPC). Further characterization of AR-V7 and its clinical… read more here.

Keywords: specific rna; detection; novel junction; rna situ ... See more keywords