Small RNA In Situ Hybridizations on Sections of Arabidopsis Embryos.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0342-0_7
Abstract: Small RNAs mediate posttranscriptional gene silencing in plants and animals. This often occurs in specific cell or tissue types and can be necessary for their differentiation. Determining small RNA (sRNA) localization patterns at cellular resolution… read more here.
Keywords: small rna; hybridizations sections; sections arabidopsis; rna situ ... See more keywords
RNA In Situ Hybridization of Detecting Cucumber Mosaic Virus in Shoots of Nicotiana benthamiana Plants.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1835-6_27
Abstract: RNA in situ hybridization, a histological technique derived from Southern blotting and northern blotting, has been an important approach in biology studies for many years. In the studies of virus-plant interactions, RNA in situ hybridization… read more here.
Keywords: cucumber mosaic; mosaic virus; rna situ; biology ... See more keywords
RNA In Situ Hybridization on Planarian Paraffin Sections.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7802-1_13
Abstract: RNA in situ hybridization techniques are an important tool for the study of gene expression patterns in freshwater planarians. Here I describe a RNA in situ hybridization method on histological paraffin sections of planarian tissue.… read more here.
Keywords: hybridization planarian; rna situ; situ hybridization; paraffin sections ... See more keywords
RNA in situ hybridization and expression of related genes regulating the accumulation of triterpenoids in Cyclocarya paliurus.
Sign Up to like & getrecommendations! Published in 2021 at "Tree physiology"
DOI: 10.1093/treephys/tpab067
Abstract: Cyclocarya paliurus, a woody medicinal species in the Juglandaceae, grows extensively in subtropical areas of China. Triterpenoids in the leaves have health-promoting effects, including hypoglycemic and hypolipidemic activities. To understand triterpenoid biosynthesis, transport, and accumulation… read more here.
Keywords: accumulation; expression; paliurus; gene ... See more keywords
RNA In Situ Hybridization of Whole-Mount Mosquito Olfactory Tissues.
Sign Up to like & getrecommendations! Published in 2022 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot107916
Abstract: This protocol presents a method for RNA in situ hybridization of whole-mounted preparations of mosquito antennae and maxillary palps. It can be adapted for other peripheral appendages such as the legs or proboscis. read more here.
Keywords: mount mosquito; situ hybridization; hybridization whole; rna situ ... See more keywords
The utility of ETV1, ETV4 and ETV5 RNA in‐situ hybridization in the diagnosis of CIC–DUX sarcomas
Sign Up to like & getrecommendations! Published in 2017 at "Histopathology"
DOI: 10.1111/his.13112
Abstract: A recently characterized group of undifferentiated small round cell sarcomas harbours fusions of the genes CIC and DUX4. Studies report a distinctive gene expression profile for these sarcomas, including expression of E26 transformation‐specific (ETS) family… read more here.
Keywords: sarcomas; etv4 etv5; utility; etv1 etv4 ... See more keywords
Evaluating the effectiveness of RNA in‐situ hybridization for detecting lung adenocarcinoma with anaplastic lymphoma kinase rearrangement
Sign Up to like & getrecommendations! Published in 2017 at "Histopathology"
DOI: 10.1111/his.13198
Abstract: An easy and rapid assay for detecting mRNA in formalin‐fixed paraffin‐embedded samples [RNA in‐situ hybridization (ISH)] has been reported recently. The aim of this study was to investigate the diagnostic accuracy of RNA ISH in… read more here.
Keywords: lung adenocarcinoma; detecting lung; anaplastic lymphoma; rna situ ... See more keywords
Novel In Situ Hybridization Assay for Chromogenic Single-Molecule Detection of Human Papillomavirus E6/E7 mRNA
Sign Up to like & getrecommendations! Published in 2023 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.03896-22
Abstract: In situ detection of viral mRNA expression in tissue samples is of great value for pathological diagnosis to access viral infection status. Unfortunately, conventional RNA in situ hybridization assays lack sensitivity and specificity for clinical… read more here.
Keywords: situ hybridization; mrna; hybridization; hybridization assay ... See more keywords
AR-V7 detection by a novel junction-specific RNA in situ hybridization (RISH) test.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Clinical Oncology"
DOI: 10.1200/jco.2017.35.6_suppl.191
Abstract: 191Background: Previous studies have reported an association between blood-based detection of androgen receptor splice variant-7 (AR-V7) and resistance to abiraterone/enzalutamide in men with metastatic castration-resistant prostate cancer (mCRPC). Further characterization of AR-V7 and its clinical… read more here.
Keywords: specific rna; detection; novel junction; rna situ ... See more keywords
Quantitative analysis of HER2 mRNA expression by RNA in situ hybridization in canine mammary gland tumors: Comparison with immunohistochemistry analysis
Sign Up to like & getrecommendations! Published in 2020 at "PLoS ONE"
DOI: 10.1371/journal.pone.0229031
Abstract: Spontaneously occurring canine mammary gland tumors share many features with human breast cancer, including biological behavior and histologic features. Compared to transgenic murine model, canine models have advantages including naturally occurring models of human diseases… read more here.
Keywords: canine mammary; canine; expression; her2 ... See more keywords
Improvement of RNA In Situ Hybridisation for Grapevine Fruits and Ovules
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24010800
Abstract: The European grapevine (Vitis vinifera L.) is one of the world’s most widely cultivated and economically important fruit crops. Seedless fruits are particularly desired for table grapes, with seedlessness resulting from stenospermocarpy being an important… read more here.
Keywords: improvement; situ hybridisation; hybridisation; grapevine ... See more keywords