Slippage at the initiation of RNA synthesis by Qβ replicase results in a periodic polyG pattern
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Letters"
DOI: 10.1002/1873-3468.14556
Abstract: The repetitive copying of template nucleotides due to transcriptional slippage has not been reported for RNA‐directed RNA polymerases of positive‐strand RNA phages. We unexpectedly observed that, with GTP as the only substrate, Qβ replicase, the… read more here.
Keywords: slippage; replicase; synthesis; initiation rna ... See more keywords
Benzamidine ML336 inhibits plus and minus strand RNA synthesis of Venezuelan equine encephalitis virus without affecting host RNA production.
Sign Up to like & getrecommendations! Published in 2019 at "Antiviral research"
DOI: 10.1016/j.antiviral.2019.104674
Abstract: Venezuelan equine encephalitis virus (VEEV) is an alphavirus that is endemic to the Americas. VEEV outbreaks occur periodically and cause encephalitis in both humans and equids. There are currently no therapeutics or vaccines for treatment… read more here.
Keywords: rna synthesis; encephalitis; veev; ml336 inhibits ... See more keywords
Template requirements of Zika RNA polymerase during in vitro RNA synthesis from the 3'-end of virus minus-strand RNA.
Sign Up to like & getrecommendations! Published in 2021 at "Biochimie"
DOI: 10.1016/j.biochi.2021.11.003
Abstract: As ZIKV continues to spread, many "unknowns" remain and research is needed to advance the understanding of this important pathogen. Viral RNA dependent-RNA polymerases (RdRp) are validated targets for inhibitors of the replication of several… read more here.
Keywords: minus strand; rna synthesis; strand rna; rna ... See more keywords
Multiply Intercalator-Substituted Cu(II) Cyclen Complexes as DNA Condensers and DNA/RNA Synthesis Inhibitors.
Sign Up to like & getrecommendations! Published in 2018 at "Inorganic chemistry"
DOI: 10.1021/acs.inorgchem.8b00027
Abstract: Many drugs that are applied in anticancer therapy such as the anthracycline doxorubicin contain DNA-intercalating 9,10-anthraquinone (AQ) moieties. When Cu(II) cyclen complexes were functionalized with up to three (2-anthraquinonyl)methyl substituents, they efficiently inhibited DNA and… read more here.
Keywords: rna synthesis; dna; multiply intercalator; dna rna ... See more keywords
RNA synthesis is modulated by G-quadruplex formation in Hepatitis C virus negative RNA strand
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-26582-3
Abstract: DNA and RNA guanine-rich oligonucleotides can form non-canonical structures called G-quadruplexes or “G4” that are based on the stacking of G-quartets. The role of DNA and RNA G4 is documented in eukaryotic cells and in… read more here.
Keywords: rna synthesis; negative rna; rna; formation ... See more keywords
Complementary Mutations in the N and L Proteins for Restoration of Viral RNA Synthesis
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Virology"
DOI: 10.1128/jvi.01417-18
Abstract: During viral RNA synthesis of a negative-strand RNA virus, the viral RNA-dependent RNA polymerase (vRdRp) must gain access to the sequestered RNA in the nucleocapsid to use it as the template, but at the same… read more here.
Keywords: sequestered rna; rna synthesis; rna; structural motif ... See more keywords
Constraints of Viral RNA Synthesis on Codon Usage of Negative-Strand RNA Virus
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Virology"
DOI: 10.1128/jvi.01775-18
Abstract: Negative-strand RNA viruses (NSVs) include the most pathogenic viruses known. New methods to monitor their evolutionary trends are urgently needed for the development of antivirals and vaccines. The protein translation machinery of the host cell… read more here.
Keywords: rna synthesis; strand rna; codon usage; rna ... See more keywords
In Vitro Primer-Based RNA Elongation and Promoter Fine Mapping of the Respiratory Syncytial Virus
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Virology"
DOI: 10.1128/jvi.01897-20
Abstract: As a major human pathogen, RSV affects 3.4 million children worldwide annually. However, no effective antivirals or vaccines are available. An in-depth mechanistic understanding of the RSV RNA synthesis machinery remains a high priority among… read more here.
Keywords: rsv; rna synthesis; rsv polymerase; virus ... See more keywords
Involvement of Actin-Regulating Factor Cofilin in the Inclusion Body Formation and RNA Synthesis of Human Parainfluenza Virus Type 3 via Interaction With the Nucleoprotein
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2019.00095
Abstract: Human parainfluenza virus type 3 (HPIV3) is one of the primary pathogens that causing severe respiratory tract diseases in newborns and infants. It could induce inclusion bodies (IBs) in infected cells. Comprised of viral nucleoprotein… read more here.
Keywords: rna synthesis; ibs formation; formation; cofilin ... See more keywords
The Curious Case of the Nidovirus Exoribonuclease: Its Role in RNA Synthesis and Replication Fidelity
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2019.01813
Abstract: Among RNA viruses, the order Nidovirales stands out for including viruses with the largest RNA genomes currently known. Nidoviruses employ a complex RNA-synthesizing machinery comprising a variety of non-structural proteins (nsps). One of the postulated… read more here.
Keywords: fidelity; rna; rna synthesis; synthesis replication ... See more keywords
NusG, an Ancient Yet Rapidly Evolving Transcription Factor
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2020.619618
Abstract: Timely and accurate RNA synthesis depends on accessory proteins that instruct RNA polymerase (RNAP) where and when to start and stop transcription. Among thousands of transcription factors, NusG/Spt5 stand out as the only universally conserved… read more here.
Keywords: transcription; rna synthesis; ancient yet; rapidly evolving ... See more keywords