The Fragile X Protein Disordered Regions Bind a Novel RNA Target.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemistry"
DOI: 10.1021/acs.biochem.2c00228
Abstract: The fragile X proteins (FXPs) are a family of RNA-binding proteins that regulate mRNA translation to promote proper neural development and cognition in mammals. Of particular interest to researchers is the fragile X mental retardation… read more here.
Keywords: fragile protein; rna target; protein disordered; target ... See more keywords
CRISPR DNA base editors with reduced RNA off-target and self-editing activities
Sign Up to like & getrecommendations! Published in 2019 at "Nature biotechnology"
DOI: 10.1038/s41587-019-0236-6
Abstract: Cytosine or adenine base editors (CBEs or ABEs) can introduce specific DNA C-to-T or A-to-G alterations1–4. However, we recently demonstrated that they can also induce transcriptome-wide guide-RNA-independent editing of RNA bases5, and created selective curbing… read more here.
Keywords: base editors; rna; target; dna base ... See more keywords
A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects
Sign Up to like & getrecommendations! Published in 2020 at "Nature Methods"
DOI: 10.1038/s41592-020-0832-x
Abstract: Cytosine base editors (CBEs) offer a powerful tool for correcting point mutations, yet their DNA and RNA off-target activities have caused concerns in biomedical applications. We describe screens of 23 rationally engineered CBE variants, which… read more here.
Keywords: rna target; cytosine base; target; dna rna ... See more keywords
Further Probing of Cu2+-Dependent PNAzymes Acting as Artificial RNA Restriction Enzymes
Sign Up to like & getrecommendations! Published in 2019 at "Molecules"
DOI: 10.3390/molecules24040672
Abstract: Peptide nucleic acid (PNA)-neocuproine conjugates have been shown to efficiently catalyse the cleavage of RNA target sequences in the presence of Cu2+ ions in a site-specific manner. These artificial enzymes are designed to force the… read more here.
Keywords: pnazyme; cleavage; rna; cu2 dependent ... See more keywords