Raman Spectroscopy Based Detection of RNA viruses in Saliva: a preliminary report
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Biophotonics"
DOI: 10.1002/jbio.202000189
Abstract: Several non‐invasive Raman spectroscopy‐based assays have been reported for rapid and sensitive detection of pathogens. We developed a novel statistical model for the detection of RNA viruses in saliva, based on an unbiased selection of… read more here.
Keywords: viruses saliva; rna viruses; rna; spectroscopy ... See more keywords
Universal Library Preparation Protocol for Efficient High-Throughput Sequencing of Double-Stranded RNA Viruses.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0138-9_14
Abstract: This chapter reports a library preparation protocol for efficient high-throughput sequencing of double-stranded RNA viruses. The protocol consists of four main steps, viz., enzyme treatment, precipitation using lithium chloride, full-length amplification of cDNAs, and tailing… read more here.
Keywords: rna viruses; double stranded; stranded rna; throughput sequencing ... See more keywords
Genome polarity of RNA viruses reflects the different evolutionary pressures shaping codon usage
Sign Up to like & getrecommendations! Published in 2018 at "Archives of Virology"
DOI: 10.1007/s00705-018-3930-7
Abstract: RNA viruses are classified by their genome polarity and replication strategies. Nucleotide composition and codon usage differ among virus groups, for instance positive-sense RNA (+ssRNA) viruses have higher GC-content than the other RNA virus groups.… read more here.
Keywords: genome polarity; rna viruses; ssrna viruses; rna ... See more keywords
Role of artificial intelligence in peptide vaccine design against RNA viruses
Sign Up to like & getrecommendations! Published in 2021 at "Informatics in Medicine Unlocked"
DOI: 10.1016/j.imu.2021.100768
Abstract: RNA viruses have high rate of replication and mutation that help them adapt and change according to their environmental conditions. Many viral mutants are the cause of various severe and lethal diseases. Vaccines, on the… read more here.
Keywords: artificial intelligence; peptide vaccine; rna; rna viruses ... See more keywords
Evaluation of cell viability dyes in antiviral assays with RNA viruses that exhibit different cytopathogenic properties.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2017.03.012
Abstract: Studies were conducted to determine the performance of four dyes in assessing antiviral activities of compounds against three RNA viruses with differing cytopathogenic properties. Dyes included alamarBlue® measured by absorbance (ALB-A) and fluorescence (ALB-F), neutral… read more here.
Keywords: cell viability; cytopathogenic properties; rna viruses; cc50 values ... See more keywords
Constructing RNA Viruses for Long-Term Transcriptional Gene Silencing.
Sign Up to like & getrecommendations! Published in 2019 at "Trends in biotechnology"
DOI: 10.1016/j.tibtech.2018.07.015
Abstract: Recent discoveries have shown that self-replicating RNA viruses can produce small RNAs (sRNAs) in host cells. Given their potential to be modified to generate short-term transgene expression without integrating viral sequences into the host genome,… read more here.
Keywords: term; rna viruses; term transcriptional; long term ... See more keywords
Meta-transcriptomics and the evolutionary biology of RNA viruses
Sign Up to like & getrecommendations! Published in 2018 at "Virus Research"
DOI: 10.1016/j.virusres.2017.10.016
Abstract: Abstract Metagenomics is transforming the study of virus evolution, allowing the full assemblage of virus genomes within a host sample to be determined rapidly and cheaply. The genomic analysis of complete transcriptomes, so-called meta-transcriptomics, is… read more here.
Keywords: virus; meta transcriptomics; biology; rna viruses ... See more keywords
Metagenomics reshapes the concepts of RNA virus evolution by revealing extensive horizontal virus transfer
Sign Up to like & getrecommendations! Published in 2018 at "Virus Research"
DOI: 10.1016/j.virusres.2017.10.020
Abstract: Abstract Virus metagenomics is a young research filed but it has already transformed our understanding of virus diversity and evolution, and illuminated at a new level the connections between virus evolution and the evolution and… read more here.
Keywords: virus evolution; evolution; rna viruses; horizontal virus ... See more keywords
Diversity of DNA and RNA Viruses in Indoor Air As Assessed via Metagenomic Sequencing.
Sign Up to like & getrecommendations! Published in 2018 at "Environmental science & technology"
DOI: 10.1021/acs.est.7b04203
Abstract: Diverse bacterial and fungal communities inhabit human-occupied buildings and circulate in indoor air; however, viral diversity in these man-made environments remains largely unknown. Here we investigated DNA and RNA viruses circulating in the air of… read more here.
Keywords: rna viruses; diversity; metagenomic sequencing; air ... See more keywords
Attenuation of RNA viruses by redirecting their evolution in sequence space
Sign Up to like & getrecommendations! Published in 2017 at "Nature Microbiology"
DOI: 10.1038/nmicrobiol.2017.88
Abstract: RNA viruses pose serious threats to human health. Their success relies on their capacity to generate genetic variability and, consequently, on their adaptive potential. We describe a strategy to attenuate RNA viruses by altering their… read more here.
Keywords: sequence space; rna; attenuation rna; rna viruses ... See more keywords
Immune priming and clearance of orally acquired RNA viruses in Drosophila
Sign Up to like & getrecommendations! Published in 2018 at "Nature Microbiology"
DOI: 10.1038/s41564-018-0265-9
Abstract: Immune responses in insects are differentially triggered depending on the infection route used by the pathogen. In most studies involving Drosophila melanogaster and viruses, infection is done by injection, while oral infection, which is probably… read more here.
Keywords: insects; oral infections; rna viruses; infection ... See more keywords