Genome-wide RNAi screen identifies novel players in human 60S subunit biogenesis including key enzymes of polyamine metabolism
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac072
Abstract: Abstract Ribosome assembly is an essential process that is linked to human congenital diseases and tumorigenesis. While great progress has been made in deciphering mechanisms governing ribosome biogenesis in eukaryotes, an inventory of factors that… read more here.
Keywords: 60s subunit; polyamine metabolism; wide rnai; enzymes polyamine ... See more keywords
An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac1084
Abstract: Abstract RNA helicases are involved in multiple steps of RNA metabolism to direct their roles in gene expression, yet their functions in pluripotency control remain largely unexplored. Starting from an RNA interference (RNAi) screen of… read more here.
Keywords: cell; rnai screen; rna helicases; pluripotency ... See more keywords
ATRT-09. IDENTIFICATION OF POTENTIAL GENETIC DRIVERS OF METHOTREXATE (MTX) RESISTANCE IN ATYPICAL TERATOID RHABDOID TUMOURS (ATRT) THROUGH A GENOME-WIDE RNAI SCREEN
Sign Up to like & getrecommendations! Published in 2021 at "Neuro-Oncology"
DOI: 10.1093/neuonc/noab090.008
Abstract: Abstract ATRT of the CNS constitute a group of rare and aggressive early childhood tumors with poor prognosis. While there are differing chemotherapeutic regimens for ATRT, high-dose MTX is a crucial component of many therapeutic… read more here.
Keywords: genome wide; wide rnai; mtx resistance; resistance ... See more keywords
Large-scale RNAi screen identified Dhpr as a regulator of mitochondrial morphology and tissue homeostasis
Sign Up to like & getrecommendations! Published in 2019 at "Science Advances"
DOI: 10.1126/sciadv.aax0365
Abstract: Dhpr regulates mitochondrial morphology by modulating S-nitrosylation of Drp1. Mitochondria are highly dynamic organelles. Through a large-scale in vivo RNA interference (RNAi) screen that covered around a quarter of the Drosophila melanogaster genes (4000 genes),… read more here.
Keywords: large scale; mitochondrial morphology; morphology; morphology tissue ... See more keywords
A trafficome-wide RNAi screen reveals deployment of early and late secretory host proteins and the entire late endo-/lysosomal vesicle fusion machinery by intracellular Salmonella
Sign Up to like & getrecommendations! Published in 2020 at "PLoS Pathogens"
DOI: 10.1371/journal.ppat.1008220
Abstract: The intracellular lifestyle of Salmonella enterica is characterized by the formation of a replication-permissive membrane-bound niche, the Salmonella-containing vacuole (SCV). As a further consequence of the massive remodeling of the host cell endosomal system, intracellular… read more here.
Keywords: fusion; salmonella; intracellular salmonella; host ... See more keywords
A Systematic RNAi Screen Reveals a Novel Role of a Spindle Assembly Checkpoint Protein BuGZ in Synaptic Transmission in C. elegans
Sign Up to like & getrecommendations! Published in 2017 at "Frontiers in Molecular Neuroscience"
DOI: 10.3389/fnmol.2017.00141
Abstract: Synaptic vesicles (SV) store various neurotransmitters that are released at the synapse. The molecular mechanisms of biogenesis, exocytosis, and endocytosis for SV, however, remain largely elusive. In this study, using Complex Object Parametric Analysis and… read more here.
Keywords: spindle assembly; checkpoint protein; screen; protein bugz ... See more keywords