Branched-chain polyamine stabilizes RNA polymerase at elevated temperatures in hyperthermophiles
Sign Up to like & getrecommendations! Published in 2019 at "Amino Acids"
DOI: 10.1007/s00726-019-02745-y
Abstract: Branched-chain polyamines (BCPAs) are unique polycations found in (hyper)thermophiles. Thermococcus kodakarensis grows optimally at 85 °C and produces the BCPA N 4 -bis(aminopropyl)spermidine by sequential addition of decarboxylated S -adenosylmethionine (dcSAM) aminopropyl groups to spermidine (SPD)… read more here.
Keywords: bcpa; rna polymerase; rnap dbp4; branched chain ... See more keywords
m6A RNA methylation regulates promoter- proximal pausing of RNA polymerase II.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular cell"
DOI: 10.1016/j.molcel.2021.06.023
Abstract: RNA polymerase II (RNAP II) pausing is essential to precisely control gene expression and is critical for development of metazoans. Here, we show that the m6A RNA modification regulates promoter-proximal RNAP II pausing in Drosophila… read more here.
Keywords: m6a rna; gene; rnap; rna polymerase ... See more keywords
Exploring the potential of T7 bacteriophage protein Gp2 as a novel inhibitor of mycobacterial RNA polymerase.
Sign Up to like & getrecommendations! Published in 2017 at "Tuberculosis"
DOI: 10.1016/j.tube.2017.07.004
Abstract: Over the past six decades, there has been a decline in novel therapies to treat tuberculosis, while the causative agent of this disease has become increasingly resistant to current treatment regimens. Bacteriophages (phages) are able… read more here.
Keywords: protein; exploring potential; gp2; rna polymerase ... See more keywords
The transcription-repair coupling factor Mfd associates with RNA polymerase in the absence of exogenous damage
Sign Up to like & getrecommendations! Published in 2018 at "Nature Communications"
DOI: 10.1038/s41467-018-03790-z
Abstract: During transcription elongation, bacterial RNA polymerase (RNAP) can pause, backtrack or stall when transcribing template DNA. Stalled transcription elongation complexes at sites of bulky lesions can be rescued by the transcription terminator Mfd. The molecular… read more here.
Keywords: transcription; mfd; rna polymerase; absence exogenous ... See more keywords
Structural mechanism of transcription inhibition by lasso peptides microcin J25 and capistruin
Sign Up to like & getrecommendations! Published in 2019 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.1817352116
Abstract: Significance Many bacteria produce antimicrobial peptides for survival under stressful conditions. Some of these antimicrobial peptides are lasso peptides, which have a unique lasso-like topology and have generated great interest as a result of their… read more here.
Keywords: lasso peptides; inhibition; peptides microcin; rnap ... See more keywords
A single mutation attenuates both the transcription termination and RNA-dependent RNA polymerase activity of T7 RNA polymerase.
Sign Up to like & getrecommendations! Published in 2021 at "RNA biology"
DOI: 10.1080/15476286.2021.1954808
Abstract: Transcription termination is one of the least understood processes of gene expression. As the prototype model for transcription studies, the single-subunit T7 RNA polymerase (RNAP) is known to respond to two types of termination signals,… read more here.
Keywords: transcription; rna polymerase; polymerase; rnap ... See more keywords
Psychrophilic phage VSW-3 RNA polymerase reduces both terminal and full-length dsRNA byproducts in in vitro transcription
Sign Up to like & getrecommendations! Published in 2022 at "RNA Biology"
DOI: 10.1080/15476286.2022.2139113
Abstract: ABSTRACT RNA research and applications are underpinned by in vitro transcription (IVT), but RNA impurities resulting from the enzymatic reagents severely impede downstream applications. To improve the stability and purity of synthesized RNA, we have… read more here.
Keywords: vsw; transcription; rnap; rna polymerase ... See more keywords
Density of σ70 promoter-like sites in the intergenic regions dictates the redistribution of RNA polymerase during osmotic stress in Escherichia coli.
Sign Up to like & getrecommendations! Published in 2019 at "Nucleic acids research"
DOI: 10.1093/nar/gkz159
Abstract: RNA polymerase (RNAP), the transcription machinery, shows dynamic binding across the genomic DNA under different growth conditions. The genomic features that selectively redistribute the limited RNAP molecules to dictate genome-wide transcription in response to environmental… read more here.
Keywords: promoter like; like sites; stress; rnap ... See more keywords
Domains within RbpA Serve Specific Functional Roles That Regulate the Expression of Distinct Mycobacterial Gene Subsets
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Bacteriology"
DOI: 10.1128/jb.00690-17
Abstract: ABSTRACT The RNA polymerase (RNAP) binding protein A (RbpA) contributes to the formation of stable RNAP-promoter open complexes (RPo) and is essential for viability in mycobacteria. Four domains have been identified in the RbpA protein,… read more here.
Keywords: mycobacterial gene; gene expression; rbpa; rnap ... See more keywords
Gene expression of RNAP II, JBP1 and JBP2 in Leishmania major exposed to antimonials, amphotericin B and paromomycin
Sign Up to like & getrecommendations! Published in 2018 at "Annals of parasitology"
DOI: 10.17420/ap6403.149
Abstract: Cutaneous leishmaniosis (CL) is treated with pentavalent antimony (SbV) as a first-line drug, while amphotericin B and paromomycin are potential alternatives in antimonial- resistant isolates. However, the mechanisms of drug resistance remain unclear. The present… read more here.
Keywords: amphotericin paromomycin; gene expression; jbp2; expression ... See more keywords