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   Articles with "rnap" as a keyword



Synthetic Genetic Circuits Enabled in Komagataella phaffii Through T7 RNAP/CRISPRa System

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recommendations! Published in 2025 at "Biotechnology Journal"

DOI: 10.1002/biot.70036

Abstract: The CRISPR activation (CRISPRa) transcriptional system has become a powerful synthetic biology tool for the regulation of endogenous gene expression, allowing for precise fine‐tuning of target genes through the simple modification of sgRNA sequences. In… read more here.

Keywords: system; genetic circuits; rnap; komagataella phaffii ... See more keywords

Branched-chain polyamine stabilizes RNA polymerase at elevated temperatures in hyperthermophiles

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recommendations! Published in 2019 at "Amino Acids"

DOI: 10.1007/s00726-019-02745-y

Abstract: Branched-chain polyamines (BCPAs) are unique polycations found in (hyper)thermophiles. Thermococcus kodakarensis grows optimally at 85 °C and produces the BCPA N 4 -bis(aminopropyl)spermidine by sequential addition of decarboxylated S -adenosylmethionine (dcSAM) aminopropyl groups to spermidine (SPD)… read more here.

Keywords: bcpa; rna polymerase; rnap dbp4; branched chain ... See more keywords

m6A RNA methylation regulates promoter- proximal pausing of RNA polymerase II.

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recommendations! Published in 2021 at "Molecular cell"

DOI: 10.1016/j.molcel.2021.06.023

Abstract: RNA polymerase II (RNAP II) pausing is essential to precisely control gene expression and is critical for development of metazoans. Here, we show that the m6A RNA modification regulates promoter-proximal RNAP II pausing in Drosophila… read more here.

Keywords: m6a rna; gene; rnap; rna polymerase ... See more keywords

Exploring the potential of T7 bacteriophage protein Gp2 as a novel inhibitor of mycobacterial RNA polymerase.

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recommendations! Published in 2017 at "Tuberculosis"

DOI: 10.1016/j.tube.2017.07.004

Abstract: Over the past six decades, there has been a decline in novel therapies to treat tuberculosis, while the causative agent of this disease has become increasingly resistant to current treatment regimens. Bacteriophages (phages) are able… read more here.

Keywords: protein; exploring potential; gp2; rna polymerase ... See more keywords

The transcription-repair coupling factor Mfd associates with RNA polymerase in the absence of exogenous damage

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recommendations! Published in 2018 at "Nature Communications"

DOI: 10.1038/s41467-018-03790-z

Abstract: During transcription elongation, bacterial RNA polymerase (RNAP) can pause, backtrack or stall when transcribing template DNA. Stalled transcription elongation complexes at sites of bulky lesions can be rescued by the transcription terminator Mfd. The molecular… read more here.

Keywords: transcription; mfd; rna polymerase; absence exogenous ... See more keywords

DNA aptamers targeting P. aeruginosa RNAP.

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recommendations! Published in 2025 at "Chemical communications"

DOI: 10.1039/d5cc00682a

Abstract: We present the first DNA aptamers designed to target the RNA polymerase (RNAP) of Pseudomonas aeruginosa. Utilizing SELEX, we identified and examined aptamers, among which the R2 aptamer demonstrated high specificity and significant binding affinity… read more here.

Keywords: aptamers targeting; rnap; dna aptamers; aeruginosa rnap ... See more keywords

Structural mechanism of transcription inhibition by lasso peptides microcin J25 and capistruin

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recommendations! Published in 2019 at "Proceedings of the National Academy of Sciences of the United States of America"

DOI: 10.1073/pnas.1817352116

Abstract: Significance Many bacteria produce antimicrobial peptides for survival under stressful conditions. Some of these antimicrobial peptides are lasso peptides, which have a unique lasso-like topology and have generated great interest as a result of their… read more here.

Keywords: lasso peptides; inhibition; peptides microcin; rnap ... See more keywords
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A single mutation attenuates both the transcription termination and RNA-dependent RNA polymerase activity of T7 RNA polymerase.

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recommendations! Published in 2021 at "RNA biology"

DOI: 10.1080/15476286.2021.1954808

Abstract: Transcription termination is one of the least understood processes of gene expression. As the prototype model for transcription studies, the single-subunit T7 RNA polymerase (RNAP) is known to respond to two types of termination signals,… read more here.

Keywords: transcription; rna polymerase; polymerase; rnap ... See more keywords

Psychrophilic phage VSW-3 RNA polymerase reduces both terminal and full-length dsRNA byproducts in in vitro transcription

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recommendations! Published in 2022 at "RNA Biology"

DOI: 10.1080/15476286.2022.2139113

Abstract: ABSTRACT RNA research and applications are underpinned by in vitro transcription (IVT), but RNA impurities resulting from the enzymatic reagents severely impede downstream applications. To improve the stability and purity of synthesized RNA, we have… read more here.

Keywords: vsw; transcription; rnap; rna polymerase ... See more keywords

Density of σ70 promoter-like sites in the intergenic regions dictates the redistribution of RNA polymerase during osmotic stress in Escherichia coli.

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recommendations! Published in 2019 at "Nucleic acids research"

DOI: 10.1093/nar/gkz159

Abstract: RNA polymerase (RNAP), the transcription machinery, shows dynamic binding across the genomic DNA under different growth conditions. The genomic features that selectively redistribute the limited RNAP molecules to dictate genome-wide transcription in response to environmental… read more here.

Keywords: promoter like; like sites; stress; rnap ... See more keywords

MoaB2, a newly identified transcription factor, binds to σA in Mycobacterium smegmatis

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recommendations! Published in 2024 at "Journal of Bacteriology"

DOI: 10.1128/jb.00066-24

Abstract: ABSTRACT In mycobacteria, σA is the primary sigma factor. This essential protein binds to RNA polymerase (RNAP) and mediates transcription initiation of housekeeping genes. Our knowledge about this factor in mycobacteria is limited. Here, we… read more here.

Keywords: mycobacterium smegmatis; moab2; mycobacterial transcription; factor ... See more keywords