Simultaneous detection of the subcellular localization of RNAs and proteins in cultured cells by combined multicolor RNA-FISH and IF.
Sign Up to like & getrecommendations! Published in 2017 at "Methods"
DOI: 10.1016/j.ymeth.2016.09.010
Abstract: Fluorescence in situ hybridization (FISH) and immunofluorescence (IF) are sensitive techniques used for detecting nucleic acids and proteins in cultured cells. However, these techniques are rarely applied together, and standard protocols are not readily compatible… read more here.
Keywords: cultured cells; proteins cultured; rna fish; multicolor rna ... See more keywords
A new chemical approach for proximity labelling of chromatin-associated RNAs and proteins with visible light irradiation.
Sign Up to like & getrecommendations! Published in 2019 at "Chemical communications"
DOI: 10.1039/c9cc06251c
Abstract: A new nucleus-localized singlet oxygen generator was designed and synthesized. Its capability to label nucleus-localized biomolecules through spatially-restricted oxidation under visible light was validated using fluorescence confocal microscopy. Western blot and RT-qPCR were performed to… read more here.
Keywords: new chemical; visible light; chromatin associated; associated rnas ... See more keywords