Knocking Down Long Noncoding RNAs Using Antisense Oligonucleotide Gapmers.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0771-8_3
Abstract: Long noncoding RNAs (lncRNAs) are a class of RNA with 200 nucleotides or longer that are not translated into protein. lncRNAs are highly abundant; a study estimates that at least four times more lncRNAs are… read more here.
Keywords: knocking long; long noncoding; using antisense; rnas using ... See more keywords
Nonradioactive Detection of Small RNAs Using Digoxigenin-Labeled Probes.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7165-7_14
Abstract: Small RNAs have been traditionally detected and quantified using small RNA blots, a modified Northern blot technique. The small RNAs are size-fractionated from the rest of the cellular RNA molecules by polyacrylamide gel electrophoresis and… read more here.
Keywords: small rna; small rnas; using digoxigenin; rnas using ... See more keywords
Trans ligation of RNAs to generate hybrid circular RNAs using highly efficient autocatalytic transcripts.
Sign Up to like & getrecommendations! Published in 2021 at "Methods"
DOI: 10.1016/j.ymeth.2021.05.009
Abstract: Circular RNAs are useful entities for various biotechnology applications, such as templating translation and binding or sequestering miRNA and RNA binding proteins. Circular RNA as highly resistant to degradation in cells and are more long-lived… read more here.
Keywords: hybrid circular; circular rnas; ligation; rnas using ... See more keywords