Robust counterselection and advanced λRed recombineering enable markerless chromosomal integration of large heterologous constructs
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DOI: 10.1093/nar/gkac649
Abstract: Abstract Despite advances in bacterial genome engineering, delivery of large synthetic constructs remains challenging in practice. In this study, we propose a straightforward and robust approach for the markerless integration of DNA fragments encoding whole… read more here.
Keywords: counterselection; markerless; integration; robust counterselection ... See more keywords