A Fast and Easy Method for Specific Detection of Circular RNA by Rolling‐Circle Amplification
Sign Up to like & getrecommendations! Published in 2019 at "Chembiochem"
DOI: 10.1002/cbic.201900514
Abstract: Circular RNAs (circRNAs) represent a new class of usually noncoding transcripts with largely unknown functions. Their research is hampered not least by the inapplicability of traditional analytical methods. Herein we describe a rapid and easy… read more here.
Keywords: rolling circle; detection; circle amplification; circular rna ... See more keywords
Non‐Invasive prenatal testing with rolling circle amplification: Real‐world clinical experience in a non‐molecular laboratory
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.24870
Abstract: Non‐invasive prenatal testing (NIPT) using cell‐free DNA (cfDNA) circulating in maternal blood provides a sensitive and specific screening technique for common fetal aneuploidies, but the high cost and workflow complexity of conventional methodologies limit its… read more here.
Keywords: prenatal testing; rolling circle; non invasive; circle amplification ... See more keywords
Cell-free DNA screening for fetal aneuploidy using the rolling circle method: a step towards NIPT simplification.
Sign Up to like & getrecommendations! Published in 2021 at "Prenatal diagnosis"
DOI: 10.1002/pd.6050
Abstract: OBJECTIVE To assess the efficacy of cell-free (cf)DNA screening for aneuploidy using the automated system based on rolling circle replication. METHODS A prospective study among women referred for invasive prenatal diagnosis between July 2018 and… read more here.
Keywords: rolling circle; free dna; cell free; dna screening ... See more keywords
Rolling Circle Transcription for the Self-Assembly of Multimeric RNAi Structures and Its Applications in Nanomedicine.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7138-1_4
Abstract: The enzymatic process of rolling circle transcription (RCT) enables self-assembly of multimeric RNAi structures from a circular DNA template. The self-assembled RNAi structures can be manipulated easily by simple base pairing rules with short DNA… read more here.
Keywords: self assembly; rnai structures; rolling circle; circle transcription ... See more keywords
A time-resolved luminescence aptasensor of ofloxacin based on rolling circle amplification and magnetic separation
Sign Up to like & getrecommendations! Published in 2020 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-020-02708-3
Abstract: A novel sensitive, competitive, and time-resolved luminescence sensor for the detection of ofloxacin (OFL) was developed in this study. The sensor used OFL-specific aptamer as a recognition molecule and rolling circle amplification (RCA) as a… read more here.
Keywords: rolling circle; time resolved; luminescence; resolved luminescence ... See more keywords
Rolling circle amplification and graphene-based sensor-on-a-chip for sensitive detection of serum circulating miRNAs.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2019.04.016
Abstract: In this study, we developed a simple multiplex miRNA detection platform based on rolling circle amplification and the fluorescence quenching property of reduced graphene oxide. The detection platform could be applied on a microfluidics chip… read more here.
Keywords: rolling circle; detection; circle amplification; platform ... See more keywords
Ultrasensitive assay based on a combined cascade amplification by nicking-mediated rolling circle amplification and symmetric strand-displacement amplification.
Sign Up to like & getrecommendations! Published in 2019 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2018.10.004
Abstract: MicroRNAs (miRNAs) play an important role in the regulation of various biological processes and have been used as potential biomarkers for biomedical research and clinical diagnosis. Here, nicking-mediated rolling circle amplification (N-RCA)/symmetric isothermal circular strand-displacement… read more here.
Keywords: strand displacement; rolling circle; nicking mediated; circle amplification ... See more keywords
Specific and simultaneous detection of micro RNA 21 and let-7a by rolling circle amplification combined with lateral flow strip.
Sign Up to like & getrecommendations! Published in 2019 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2018.12.040
Abstract: Owing to the pivotal function in post transcriptional gene modification, miRNA biomarkers are playing crucial role in tracking and diagnosing various forms of tumors in a short time period. Hence, the need to develop simple,… read more here.
Keywords: detection; rolling circle; circle amplification; lateral flow ... See more keywords
Molecular inversion probe-rolling circle amplification with single-strand poly-T luminescent copper nanoclusters for fluorescent detection of single-nucleotide variant of SMN gene in diagnosis of spinal muscular atrophy.
Sign Up to like & getrecommendations! Published in 2020 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2020.04.026
Abstract: In this study, a simple fluorescent detection of survival motor neuron gene (SMN) in diagnosis of spinal muscular atrophy (SMA) based on nucleic acid amplification test and the poly-T luminescent copper nanoclusters (CuNCs) was established.… read more here.
Keywords: detection; diagnosis; rolling circle; circle amplification ... See more keywords
Rolling-Circle and Strand-displacement Mechanisms for Non-enzymatic RNA Replication at the Time of the Origin of Life.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of theoretical biology"
DOI: 10.1016/j.jtbi.2021.110822
Abstract: It is likely that RNA replication began non-enzymatically, and that polymerases were later selected to speed up the process. We consider replication mechanisms in modern viruses and ask which of these is possible non-enzymatically, using… read more here.
Keywords: strand displacement; rolling circle; rna; strand ... See more keywords
Simple rolling circle amplification colorimetric assay based on pH for target DNA detection.
Sign Up to like & getrecommendations! Published in 2019 at "Talanta"
DOI: 10.1016/j.talanta.2019.04.003
Abstract: Detection and identification of DNA by PCR has opened tremendous possibilities and allows detection of minute quantities of DNA highly specifically. However, PCR remains confined to laboratory settings because of the need of thermocyclers and… read more here.
Keywords: dna; circle amplification; colorimetric assay; rolling circle ... See more keywords