Recombinase polymerase amplification-lateral flow (RPA-LF) assay combined with immunomagnetic separation for rapid visual detection of Vibrio parahaemolyticus in raw oysters
Sign Up to like & getrecommendations! Published in 2020 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-020-02532-9
Abstract: This study was the first attempt to optimize a recombinase polymerase amplification (RPA) and lateral flow (LF) assay combined with immunomagnetic separation (IMS) for the detection of Vibrio parahaemolyticus in raw oysters. The newly developed… read more here.
Keywords: detection; polymerase amplification; recombinase polymerase; rpa assay ... See more keywords
Development of propidium monoazide-recombinase polymerase amplification (PMA-RPA) assay for rapid detection of Streptococcus pyogenes and Streptococcus agalactiae.
Sign Up to like & getrecommendations! Published in 2018 at "Molecular and cellular probes"
DOI: 10.1016/j.mcp.2018.08.007
Abstract: Streptococcus pyogenes (Group A Streptococcus, GAS) and Streptococcus agalactiae (Group B Streptococcus, GBS) are common pathogens that threaten public health. In this study, a double recombinase polymerase (RPA) amplification assay was developed to rapidly detect… read more here.
Keywords: pma; streptococcus pyogenes; streptococcus agalactiae; rpa assay ... See more keywords
A novel recombinase polymerase amplification (RPA) assay for the rapid isothermal detection of Neospora caninum in aborted bovine fetuses.
Sign Up to like & getrecommendations! Published in 2018 at "Veterinary parasitology"
DOI: 10.1016/j.vetpar.2018.06.004
Abstract: The development of a method to rapidly diagnose Neospora caninum infection is highly desirable. Recombinase polymerase amplification (RPA), combined with lateral flow (LF) strips, is a novel approach to rapidly amplify and visualize DNA. We… read more here.
Keywords: detection; dna; caninum; rpa assay ... See more keywords
Endpoint Recombinase Polymerase Amplification (RPA) Assay for Enumeration of Thiocyanate-degrading Bacteria
Sign Up to like & getrecommendations! Published in 2022 at "Microbes and Environments"
DOI: 10.1264/jsme2.me21073
Abstract: An endpoint recombination amplification reaction (RPA) assay for assessing the abundance of the gene encoding thiocyanate dehydrogenase (TcDH) in Thiohalobacter has been developed. The RPA reaction was performed at 37°C for 30 min, terminated by… read more here.
Keywords: thiocyanate degrading; rpa assay; amplification; rpa ... See more keywords
Clinical Validation of a Rapid Variant-Proof RT-RPA Assay for the Detection of SARS-CoV-2
Sign Up to like & getrecommendations! Published in 2022 at "Diagnostics"
DOI: 10.3390/diagnostics12051263
Abstract: The COVID-19 pandemic has unveiled a pressing need to expand the diagnostic landscape to permit high-volume testing in peak demand. Rapid nucleic acid testing based on isothermal amplification is a viable alternative to real-time reverse… read more here.
Keywords: variant proof; rpa assay; validation rapid; clinical validation ... See more keywords
Laboratory Evaluation of a Basic Recombinase Polymerase Amplification (RPA) Assay for Early Detection of Schistosoma japonicum
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DOI: 10.3390/pathogens11030319
Abstract: Early detection of Schistosoma japonicum (S. japonicum) within its intermediate and definitive hosts is crucial for case finding and disease surveillance, especially in low-endemic areas. Recombinase polymerase amplification (RPA) has many advantages over traditional methods… read more here.
Keywords: early detection; japonicum; rpa assay; infection ... See more keywords
Development of a Real-Time Recombinase Polymerase Amplification Assay for the Rapid Detection of African Swine Fever Virus Genotype I and II
Sign Up to like & getrecommendations! Published in 2022 at "Pathogens"
DOI: 10.3390/pathogens11040439
Abstract: African swine fever (ASF) is a contagious viral disease in pigs and wild boars which poses a major threat to the pig industry. Rapid and accurate diagnosis is necessary to control ASF. Hence, we developed… read more here.
Keywords: time; polymerase; rpa assay; time rpa ... See more keywords