Effects of intragenomic polymorphism in the SSU rRNA gene on estimating marine microeukaryotic diversity: A test for ciliates using single‐cell high‐throughput DNA sequencing
Sign Up to like & getrecommendations! Published in 2019 at "Limnology and Oceanography: Methods"
DOI: 10.1002/lom3.10330
Abstract: Diversity assessments using the small subunit (SSU) rRNA gene allow for rapid assessments of microeukaryotic communities. Yet, for microeukaryotes, only limited information is available on the extent to which sequence variations within individual genomes exist… read more here.
Keywords: rrna gene; diversity; ssu rrna; cell ... See more keywords
16S rRNA gene sequencing reveals altered composition of gut microbiota in individuals with kidney stones
Sign Up to like & getrecommendations! Published in 2018 at "Urolithiasis"
DOI: 10.1007/s00240-018-1037-y
Abstract: Nephrolithiasis is a common urological disease with high prevalence and recurrence rates. Characterizing gut microbiome profiles of nephrolithiasis patients may provide valuable insights and potential biomarkers for the disease. Therefore, we explored the relation between… read more here.
Keywords: nephrolithiasis patients; gut; kidney stones; nephrolithiasis ... See more keywords
High-Throughput Sequencing of the 16S rRNA Gene as a Survey to Analyze the Microbiomes of Free-Living Ciliates Paramecium
Sign Up to like & getrecommendations! Published in 2019 at "Microbial Ecology"
DOI: 10.1007/s00248-019-01321-x
Abstract: Ciliates are the largest group of ubiquitous aquatic bacterivorous protists, and many species are easily cultivated. However, only few studies reported prokaryotic communities naturally associated with ciliate cells. Herein, we analyzed the microbiome composition of… read more here.
Keywords: 16s rrna; throughput sequencing; rrna gene; high throughput ... See more keywords
Comparison of Deep-Sea Picoeukaryotic Composition Estimated from the V4 and V9 Regions of 18S rRNA Gene with a Focus on the Hadal Zone of the Mariana Trench.
Sign Up to like & getrecommendations! Published in 2021 at "Microbial ecology"
DOI: 10.1007/s00248-021-01747-2
Abstract: Diversity of microbial eukaryotes is estimated largely based on sequencing analysis of the hypervariable regions of 18S rRNA genes. But the use of different regions of 18S rRNA genes as molecular markers may generate bias… read more here.
Keywords: mariana trench; 18s rrna; regions 18s; deep sea ... See more keywords
Novel primers for 16S rRNA gene-based archaeal and bacterial community analysis in oceanic trench sediments.
Sign Up to like & getrecommendations! Published in 2022 at "Applied microbiology and biotechnology"
DOI: 10.1007/s00253-022-11893-3
Abstract: High-throughput sequencing of the 16S ribosomal RNA (16S rRNA) gene has been successfully applied to explore the microbial structure and dynamics in various environments. The distinctive microbial communities in oceanic trench sediments are expected because… read more here.
Keywords: trench sediments; rrna gene; novel primers; 16s rrna ... See more keywords
Geographical Distribution and Identification of Phytoplasma Strain Associated with Sugarcane White Leaf Disease in Sri Lanka
Sign Up to like & getrecommendations! Published in 2021 at "Sugar Tech"
DOI: 10.1007/s12355-021-00980-w
Abstract: Sugarcane white leaf (SCWL) is one of the major sugarcane diseases in the Asian continent associated with phytoplasmas. It occurs in all sugarcane-growing areas of Sri Lanka, causing substantial economic losses. However, subgroup level identification… read more here.
Keywords: 16s rrna; white leaf; rrna gene; sri lanka ... See more keywords
Novel Anaplasma and Ehrlichia organisms infecting the wildlife of two regions of the Brazilian Amazon.
Sign Up to like & getrecommendations! Published in 2017 at "Acta tropica"
DOI: 10.1016/j.actatropica.2017.07.006
Abstract: During 2009-2012, wild animals were sampled in the Amazon biome of Brazil. Animal tissues and blood were tested by polymerase chain reaction (PCR) assays targeting DNA of the bacterial family Anaplasmataceae (genera Anaplasma, Ehrlichia, Wolbachia)… read more here.
Keywords: ehrlichia; anaplasma ehrlichia; 16s rrna; anaplasma ... See more keywords
Distribution and phylogeny of Brachyspira spp. in human intestinal spirochetosis revealed by FISH and 16S rRNA-gene analysis.
Sign Up to like & getrecommendations! Published in 2017 at "Anaerobe"
DOI: 10.1016/j.anaerobe.2017.03.012
Abstract: During six years as German National Consultant Laboratory for Spirochetes we investigated 149 intestinal biopsies from 91 patients, which were histopathologically diagnosed with human intestinal spirochetosis (HIS), using fluorescence in situ hybridization (FISH) combined with… read more here.
Keywords: brachyspira; brachyspira spp; histopathology; 16s rrna ... See more keywords
Effect of dietary corn gluten inclusion on rumen fermentation, microbiota and methane emissions in goats
Sign Up to like & getrecommendations! Published in 2020 at "Animal Feed Science and Technology"
DOI: 10.1016/j.anifeedsci.2019.114314
Abstract: The study was to evaluate replacing corn meal (CM) by corn gluten (CG) on intake, digestibility, ruminal fermentation, CH4 emissions and microbial abundance in goats. The experiment was performed using randomized block design with 2… read more here.
Keywords: fed diet; corn; goats fed; ch4 ... See more keywords
First insight into the environmental microbial communities associated with potentially pathogenic strains in pond cultured tilapia (Oreochromis niloticus) at various growth stages based on 16S, 18S, and ITS2 rRNA gene amplicons sequencing
Sign Up to like & getrecommendations! Published in 2021 at "Aquaculture"
DOI: 10.1016/j.aquaculture.2020.736007
Abstract: Abstract The tilapias have become one of the most essential and commercial freshwater aquatic products of China. Here, 16S, 18S, and ITS2 rRNA genes were used through high-throughput sequencing (HTS) to investigate the microbiome from… read more here.
Keywords: its2 rrna; pond cultured; rrna gene; growth stages ... See more keywords
18S rRNA gene sequence-structure phylogeny of the Trypanosomatida (Kinetoplastea, Euglenozoa) with special reference to Trypanosoma.
Sign Up to like & getrecommendations! Published in 2021 at "European journal of protistology"
DOI: 10.1016/j.ejop.2021.125824
Abstract: Parasites of the order Trypanosomatida are known due to their medical relevance. Despite the progress made in the past decades on understanding the evolution of this group of organisms, there are still many open questions… read more here.
Keywords: 18s rrna; sequence structure; rrna gene;