CRISPR/Cas9-mediated genome editing in Penicillium oxalicum and Trichoderma reesei using 5S rRNA promoter-driven guide RNAs.
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DOI: 10.1007/s10529-020-03024-7
Abstract: OBJECTIVE To construct convenient CRISPR/Cas9-mediated genome editing systems in industrial enzyme-producing fungi Penicillium oxalicum and Trichoderma reesei. RESULTS Employing the 5S rRNA promoter from Aspergillus niger for guide RNA expression, the β-glucosidase gene bgl2 in… read more here.
Keywords: genome editing; oxalicum; crispr cas9; rrna promoter ... See more keywords