Quantification and Discrimination of Viable and Dead Escherichia coli O157:H7 Cells from Chicken Without Enrichment by Ethidium Bromide Monoazide Real-time Loop-Mediated Isothermal Amplification
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DOI: 10.1007/s12161-017-1092-y
Abstract: In this study, a rapid and sensitive method of real-time loop-mediated isothermal amplification (Rti-LAMP) assays was developed for quantification and discrimination of viable and heat-killed E. coli O157:H7 cells treated with low concentration of ethidium… read more here.
Keywords: real time; coli o157; value; cfu ... See more keywords
Characterization and Kinetic Study of Bentonite-Coated Activated Carbon for Adsorption of DNA Polymerase Inhibitors to Improve the Detection Sensitivity of Salmonella Derived from Vegetables by Rti-LAMP
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DOI: 10.1007/s12161-020-01814-3
Abstract: Bentonite-coated activated carbon (BCAC) as an effective adsorbent can absorb soluble impurities in food to improve the detection sensitivity of Rti-LAMP assay. In the present study, BCAC was optimally prepared and characterized by SEM-EDS, FTIR,… read more here.
Keywords: adsorption; detection; rti lamp; activated carbon ... See more keywords
Real‐time loop‐mediated isothermal amplification assays combined with ethidium monoazide bromide and bentonite coated activated carbon for rapid and sensitive detection of viableEscherichia coliO157:H7 from milk without enrichment
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DOI: 10.1111/jfs.12677
Abstract: A real‐time loop‐mediated isothermal amplification (Rti‐LAMP) DNA assay was developed for rapid and sensitive detection of viable Escherichia coli O157:H7 in milk by combining with ethidium monoazide bromide (EMA) and bentonite coated activated carbon (BCAC)… read more here.
Keywords: rti lamp; milk; coli o157; detection ... See more keywords