Rubisco Engineering by Plastid Transformation and Protocols for Assessing Expression.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1472-3_10
Abstract: The assimilation of CO2 within chloroplasts is catalyzed by the bifunctional enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase, Rubisco. Within higher plants the Rubisco large subunit gene, rbcL, is encoded in the plastid genome, while the Rubisco small subunit… read more here.
Keywords: plastid transformation; rubisco engineering; engineering plastid; rubisco ... See more keywords
Rubisco mutants of Chlamydomonas reinhardtii display divergent photosynthetic parameters and lipid allocation
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8322-5
Abstract: Photosynthesis and lipid allocation were investigated in Rubisco small subunit mutants of the microalga Chlamydomonas reinhardtii. Comparative analyses were undertaken with cells grown photoheterotrophically under sulphur-replete or sulphur-depleted conditions. The Y67A Rubisco mutant, which has… read more here.
Keywords: lipid allocation; mutants chlamydomonas; rubisco mutants; rubisco ... See more keywords
Intracellular position of mitochondria in mesophyll cells differs between C3 and C4 grasses
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Plant Research"
DOI: 10.1007/s10265-017-0947-z
Abstract: In C3 plants, part of the CO2 fixed during photosynthesis in chloroplasts is released from mitochondria during photorespiration by decarboxylation of glycine via glycine decarboxylase (GDC), thereby reducing photosynthetic efficiency. The apparent positioning of most… read more here.
Keywords: intracellular position; mitochondria mesophyll; mesophyll cells; rubisco ... See more keywords
A short history of RubisCO: the rise and fall (?) of Nature's predominant CO2 fixing enzyme.
Sign Up to like & getrecommendations! Published in 2018 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2017.07.017
Abstract: Ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) is arguably one of the most abundant proteins in the biosphere and a key enzyme in the global carbon cycle. Although RubisCO has been intensively studied, its evolutionary origins and rise as… read more here.
Keywords: fixing enzyme; co2 fixing; short history; nature ... See more keywords
Canopy height affects the allocation of photosynthetic carbon and nitrogen in two deciduous tree species under elevated CO2.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of plant physiology"
DOI: 10.1016/j.jplph.2021.153584
Abstract: Down-regulation of leaf N and Rubisco under elevated CO2 (eCO2) are accompanied by increased non-structural carbohydrates (NSC) due to the sink-source imbalance. Here, to investigate whether the canopy position affects the down-regulation of Rubisco, we… read more here.
Keywords: canopy position; elevated co2; canopy; co2 canopy ... See more keywords
Mechanism of Enzyme Repair by the AAA+ Chaperone Rubisco Activase.
Sign Up to like & getrecommendations! Published in 2017 at "Molecular cell"
DOI: 10.1016/j.molcel.2017.07.004
Abstract: How AAA+ chaperones conformationally remodel specific target proteins in an ATP-dependent manner is not well understood. Here, we investigated the mechanism of the AAA+ protein Rubisco activase (Rca) in metabolic repair of the photosynthetic enzyme… read more here.
Keywords: mechanism; rca; rubisco activase; enzyme repair ... See more keywords
Hybrid Rubisco with Complete Replacement of Rice Rubisco Small Subunits by Sorghum Counterparts Confers C4-Plant-like High Catalytic Activity.
Sign Up to like & getrecommendations! Published in 2020 at "Molecular plant"
DOI: 10.1016/j.molp.2020.08.012
Abstract: Photosynthetic rate at the present atmospheric condition is limited by the CO2-fixing-enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) because of its extremely low catalytic rate (kcat) and poor affinity for CO2 (Kc) and specificity for CO2 (Sc/o). Rubisco… read more here.
Keywords: rice; plant; rbcs; sorghum ... See more keywords
Potential improvement of photosynthetic CO2 assimilation in crops by exploiting the natural variation in the temperature response of Rubisco catalytic traits.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in plant biology"
DOI: 10.1016/j.pbi.2019.05.002
Abstract: The enhancement of the photosynthetic capacity of crops by the expression of more efficient Rubisco versions has been a main target in the field of plant photosynthesis improvement. However, such an increase in the photosynthetic… read more here.
Keywords: temperature; improvement photosynthetic; co2; potential improvement ... See more keywords
Ribulose-1,5-bisphosphate carboxylase as a sustainable and promising plant source of bioactive peptides for food applications
Sign Up to like & getrecommendations! Published in 2017 at "Trends in Food Science and Technology"
DOI: 10.1016/j.tifs.2017.09.001
Abstract: Abstract Background Plant proteins are well-known precursors of bioactive peptides. In translating the peptides into functional foods, the protein sources need to be sustainable and readily available. Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is the major enzyme in… read more here.
Keywords: plant; source; ribulose bisphosphate; bioactive peptides ... See more keywords
Nucleotide Dependence of Subunit Rearrangements in Short-Form Rubisco Activase from Spinach.
Sign Up to like & getrecommendations! Published in 2017 at "Biochemistry"
DOI: 10.1021/acs.biochem.7b00574
Abstract: Higher-plant Rubisco activase (Rca) plays a critical role in regulating the activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). In vitro, Rca is known to undergo dynamic assembly-disassembly processes, with several oligomer stoichiometries coexisting over a broad concentration… read more here.
Keywords: form; nucleotide dependence; rubisco activase; subunit ... See more keywords
Complex Chaperone Dependence of Rubisco Biogenesis.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemistry"
DOI: 10.1021/acs.biochem.8b00132
Abstract: Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), a ∼530 kDa complex of 8 large (RbcL) and 8 small subunits (RbcS), mediates the fixation of atmospheric CO2 into usable sugars during photosynthesis. Despite its fundamental role, Rubisco is a remarkably… read more here.
Keywords: chaperone dependence; dependence rubisco; complex chaperone; rubisco biogenesis ... See more keywords