Conditional Single Vector CRISPR/SaCas9 Viruses for Efficient Mutagenesis in the Adult Mouse Nervous System
Sign Up to like & getrecommendations! Published in 2020 at "Cell reports"
DOI: 10.1016/j.celrep.2020.02.092
Abstract: SUMMARY Mice engineered for conditional, cell type-specific gene inactivation have dominated the field of mouse genetics because of the high efficiency of Cre-loxP-mediated recombination. Recent advances in CRISPR/Cas9 technologies have provided alternatives for rapid gene… read more here.
Keywords: single vector; mutagenesis; vector; sacas9 ... See more keywords
Improvement of muscular atrophy by AAV–SaCas9-mediated myostatin gene editing in aged mice
Sign Up to like & getrecommendations! Published in 2020 at "Cancer Gene Therapy"
DOI: 10.1038/s41417-020-0178-7
Abstract: Muscle mass and area usually decrease with age, and this phenomenon is known as sarcopenia. This age-related atrophy correlates with insufficient levels of muscle cells differentiate and proliferate regulated by the TGF-β signaling pathway and… read more here.
Keywords: gene editing; muscle; sacas9; aged mice ... See more keywords
High-fidelity KKH variant of Staphylococcus aureus Cas9 nucleases with improved base mismatch discrimination
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkab1291
Abstract: Abstract The Cas9 nuclease from Staphylococcus aureus (SaCas9) holds great potential for use in gene therapy, and variants with increased fidelity have been engineered. However, we find that existing variants have not reached the greatest… read more here.
Keywords: fidelity; kkh; sacas9; staphylococcus aureus ... See more keywords
Full-Length Model of SaCas9-sgRNA-DNA Complex in Cleavage State
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24021204
Abstract: Staphylococcus aureus Cas9 (SaCas9) is a widely used genome editing tool. Understanding its molecular mechanisms of DNA cleavage could effectively guide the engineering optimization of this system. Here, we determined the first cryo-electron microscopy structure… read more here.
Keywords: cleavage; sacas9 sgrna; sacas9; model ... See more keywords