Combinatorial use of disulfide bridges and native sulfur-SAD phasing for rapid structure determination of coiled-coils
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DOI: 10.1042/bsr20181073
Abstract: Coiled-coils are ubiquitous protein–protein interaction motifs found in many eukaryotic proteins. The elongated, flexible and often irregular nature of coiled-coils together with their tendency to form fibrous arrangements in crystals imposes challenges on solving the… read more here.
Keywords: sulfur sad; coiled coils; sad phasing; combinatorial use ... See more keywords
Sulfur-SAD phasing from microcrystals utilizing low-energy X-rays
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DOI: 10.1107/s2052252519008698
Abstract: A practical approach for obtaining S-SAD data from native protein microcrystals with low-wavelength synchrotron radiation [Guo et al. (2019), IUCrJ, 6, 532–542] is presented in this issue of IUCrJ. read more here.
Keywords: utilizing low; low energy; sad phasing; phasing microcrystals ... See more keywords
Multi-crystal native-SAD phasing at 5 keV with a helium environment
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DOI: 10.1107/s205225252200971x
Abstract: Low-energy native-SAD phasing using a helium path could be used more routinely for solving challenging membrane protein structures. read more here.
Keywords: multi crystal; sad phasing; kev helium; phasing kev ... See more keywords
Cadmium SAD phasing at CuKα wavelength
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DOI: 10.12688/f1000research.17694.1
Abstract: Single-wavelength anomalous diffraction (SAD) is the most common method for de novo elucidation of macromolecular structures by X-ray crystallography. It requires an anomalous scatterer in a crystal to calculate phases. A recent study by Panneerselvam… read more here.
Keywords: cadmium sad; sad phasing; cadmium; phasing cuk ... See more keywords