Amplification-free detection of microRNAs via a rapid microarray-based sandwich assay
Sign Up to like & getrecommendations! Published in 2017 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-017-0298-6
Abstract: The detection and profiling of microRNAs are of great interest in disease diagnosis and prognosis. In this paper, we present a method for the rapid amplification-free detection of microRNAs from total RNA samples. In a… read more here.
Keywords: detection; sandwich assay; free detection; amplification free ... See more keywords
Construction of DNA-NanoLuc luciferase conjugates for DNA aptamer-based sandwich assay using Rep protein
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology Letters"
DOI: 10.1007/s10529-018-02641-7
Abstract: ObjectiveWe developed a DNA-NanoLuc luciferase (NnaoLuc) conjugates for DNA aptamer-based sandwich assay using the catalytic domain of the replication initiator protein derived from porcine circovirus type 2 (pRep).ResultsFor construction of DNA aptamer and NanoLuc conjugate… read more here.
Keywords: sandwich assay; based sandwich; aptamer based; dna nanoluc ... See more keywords
Determination of protein tyrosine kinase-7 concentration using electrocatalytic reaction and an aptamer-antibody sandwich assay platform
Sign Up to like & getrecommendations! Published in 2021 at "Catalysis Today"
DOI: 10.1016/j.cattod.2019.05.029
Abstract: Abstract An aptamer-antibody sandwich assay for the voltammetric determination of protein tyrosine kinase-7 (PTK7) concentrations in buffer and human serum solutions was developed. A DNA aptamer specific to PTK7 was covalently immobilized onto gold nanoparticles… read more here.
Keywords: aptamer antibody; ptk7; antibody; concentration ... See more keywords
A novel electrochemical biosensor with molecularly imprinted polymers and aptamer-based sandwich assay for determining amyloid-β oligomer
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Electroanalytical Chemistry"
DOI: 10.1016/j.jelechem.2020.114017
Abstract: Abstract Amyloid-β oligomers (AβOs) are highly toxic species involved in Alzheimer's disease (AD). Hence, a reliable detection method for AβOs, which are promising potential therapeutic targets and biomarkers for AD, is of great significance for… read more here.
Keywords: detection; sandwich assay; biosensor; electrochemical biosensor ... See more keywords
Probing Queuosine Modifications of Transfer RNA in Single Living Cells via Plasmonic Affinity Sandwich Assay.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c02784
Abstract: Queuosine (Q) modification on tRNA plays an essential role in protein synthesis, participating in many tRNA functions such as folding, stability, and decoding. Appropriate analytical tools for the measurement of tRNA Q modifications are essential… read more here.
Keywords: affinity sandwich; affinity; sandwich assay; plasmonic affinity ... See more keywords
Aptamer-Based Sandwich Assay for Measurement of Thymidine Kinase 1 in Serum of Cancerous Patients.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemistry"
DOI: 10.1021/acs.biochem.8b01284
Abstract: Thymidine kinase 1 (TK1) is traditionally a serum biomarker that is elevated in the early stages of malignancies. The diagnostic and prognostic role of TK1 for screening and monitoring human malignancies has recently been investigated.… read more here.
Keywords: sandwich assay; cancerous patients; tk1; aptamer ... See more keywords
Enzyme-linked aptamer-based sandwich assay (ELASA) for detecting Plasmodium falciparum lactate dehydrogenase, a malarial biomarker
Sign Up to like & getrecommendations! Published in 2022 at "RSC Advances"
DOI: 10.1039/d2ra03796c
Abstract: Herein, we report a sensitive and selective enzyme-linked aptamer-based sandwich assay (ELASA) to detect Plasmodium falciparum lactate dehydrogenase (PfLDH), which is an attractive biomarker for malaria diagnosis and antimalarial medication. We performed the sandwich assay… read more here.
Keywords: pfldh; enzyme linked; linked aptamer; sandwich assay ... See more keywords
Gold-aptamer modified dielectrode silica surface for hepatitis C virus core antigen sandwich assay
Sign Up to like & getrecommendations! Published in 2023 at "Materials Express"
DOI: 10.1166/mex.2023.2348
Abstract: Hepatitis C virus (HCV) cause both chronic and acute hepatitis, depends on the severity from mild to serious illness, leads a lifelong illness, such as liver cancer and liver cirrhosis. Chronic HCV is generally a… read more here.
Keywords: core antigen; sandwich assay; hepatitis virus; hepatitis ... See more keywords
Aptamer-Based Sandwich Assay Formats for Detection and Discrimination of Human High- and Low-Molecular-Weight uPA for Cancer Prognosis and Diagnosis
Sign Up to like & getrecommendations! Published in 2022 at "Cancers"
DOI: 10.3390/cancers14215222
Abstract: Simple Summary Urokinase-type plasminogen activator (urokinase, uPA) is a widely discussed biomarker for cancer prognosis and diagnosis. The gold standard for the determination of protein biomarkers in physiological samples is the enzyme-linked immunosorbent assay (ELISA).… read more here.
Keywords: prognosis diagnosis; sandwich assay; molecular weight;