Development of a sensitive sandwich-ELISA assay for reliable detection of fish residues in foods.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2021.114448
Abstract: A new sandwich-type Enzyme-Linked Immunosorbent Assay (ELISA) method was developed based on goat IgG as capturing antibody and rabbit IgG as detecting antibody targeting soluble antigenic fish proteins in foods as detection targets. The assay… read more here.
Keywords: detection; development sensitive; fish residues; sandwich elisa ... See more keywords
An ultra-specific and sensitive sandwich ELISA for imatinib using two anti-imatinib antibodies.
Sign Up to like & getrecommendations! Published in 2017 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2017.03.034
Abstract: The development of an immunoassay for a low-molecular-weight drug first requires the identification of specific antibodies that do not cross-react with the drug's metabolites. If two antibodies can simultaneously recognize the entire structure of the… read more here.
Keywords: anti imatinib; sandwich elisa; drug; imatinib antibodies ... See more keywords
Development of a monoclonal sandwich ELISA for direct detection of bluetongue virus 8 in infected animals.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2017.02.003
Abstract: Bluetongue is an infectious viral disease which can cause mortality in affected ruminants, and tremendous economic damage via impacts upon fertility, milk production and the quality of wool. The disease is caused by bluetongue virus… read more here.
Keywords: detection; sandwich elisa; btv; virus ... See more keywords
Chicken IgY-based coproantigen capture ELISA for diagnosis of human opisthorchiasis.
Sign Up to like & getrecommendations! Published in 2017 at "Parasitology international"
DOI: 10.1016/j.parint.2015.10.011
Abstract: Diagnosis of Opisthorchis viverrini infection by conventional stool examination is increasingly difficult due to the low intensity of the infection after several rounds of control programmes in endemic regions as well as coinfections with intestinal… read more here.
Keywords: opisthorchiasis; diagnosis; coproantigen; sandwich elisa ... See more keywords
Development of a sandwich ELISA for the thrombin light chain identified by serum proteome analysis
Sign Up to like & getrecommendations! Published in 2017 at "Practical Laboratory Medicine"
DOI: 10.1016/j.plabm.2017.04.004
Abstract: We previously identified novel biomarker candidates in biliary tract cancer (BTC) using serum proteome analysis. Among several candidates, we focused on thrombin light chain which is a 4204 Da peptide as the most promising biomarker… read more here.
Keywords: serum proteome; thrombin light; sandwich elisa; light chain ... See more keywords
Immunodiagnosis of paramphistomosis using monoclonal antibody-based sandwich ELISA for detection of Paramphistomum gracile circulating 16 kDa antigen
Sign Up to like & getrecommendations! Published in 2017 at "Parasitology"
DOI: 10.1017/s003118201600264x
Abstract: SUMMARY In this study, we have produced a monoclonal antibody (MoAb) against 16 kDa antigen of Paramphistomum gracile (16 kDaAgPg), and developed an accurate sandwich enzyme-linked immunosorbent assay (sandwich ELISA) for the detection of circulating… read more here.
Keywords: detection; sandwich elisa; gracile; antigen ... See more keywords
New highly sensitive sandwich ELISA system for soluble endoglin quantification in different biological fluids
Sign Up to like & getrecommendations! Published in 2018 at "Scandinavian Journal of Clinical and Laboratory Investigation"
DOI: 10.1080/00365513.2018.1516892
Abstract: Abstract Soluble endoglin (sEng) is a fragment of a membrane-associated receptor (CD105) expressed on endothelial cells, mesenchymal stem cells and trophoblast cells. It is considered as a regulatory factor involved in the development of preeclampsia… read more here.
Keywords: new elisa; sandwich elisa; seng; soluble endoglin ... See more keywords
Development of a double‐antibody sandwich ELISA for sensitive detection of Yersinia pestis
Sign Up to like & getrecommendations! Published in 2019 at "Microbiology and Immunology"
DOI: 10.1111/1348-0421.12751
Abstract: We developed a biotin–streptavidin‐based sandwich ELISA for the sensitive and specific detection of Yersinia pestis. In this assay, the F1 capsular protein and Y. pestis were captured by anti‐F1 mouse monoclonal antibody followed by detection… read more here.
Keywords: detection; elisa sensitive; pestis; antibody ... See more keywords
A newly developed glucagon sandwich ELISA is useful for more accurate glucagon evaluation than the currently used sandwich ELISA in subjects with elevated plasma proglucagon‐derived peptide levels
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Diabetes Investigation"
DOI: 10.1111/jdi.13986
Abstract: Glucagon, a peptide hormone produced from proglucagon, is involved in the pathophysiology of diabetes. Plasma glucagon levels are currently measured by sandwich enzyme‐linked immunosorbent assay (ELISA), but the currently used sandwich ELISA cross‐reacts with proglucagon‐derived… read more here.
Keywords: used sandwich; sandwich elisa; currently used; glucagon ... See more keywords
Dual monoclonal antibody-based sandwich ELISA for detection of in vitro packaged Ebola virus
Sign Up to like & getrecommendations! Published in 2018 at "Diagnostic Pathology"
DOI: 10.1186/s13000-018-0773-1
Abstract: BackgroundRapid transmission and high mortality of Ebola virus disease (EVD) highlight a urgent need of large scale, convenient and effective measure for Ebola virus screening. Application of monoclonal antibodies (mAbs) are crucial for establishment of… read more here.
Keywords: detection; two mabs; ebola virus; sandwich elisa ... See more keywords
Double antibody pairs sandwich-ELISA (DAPS-ELISA) detects Acidovorax citrulli serotypes with broad coverage
Sign Up to like & getrecommendations! Published in 2020 at "PLoS ONE"
DOI: 10.1371/journal.pone.0237940
Abstract: Acidovorax citrulli, a seedborne bacterium and quarantine pest, causes the devastating bacterial fruit blotch disease in cucurbit plants. Immunological assays such as ELISA are widely used in routine field inspections for this bacterium. However, to… read more here.
Keywords: elisa daps; sandwich elisa; citrulli strains; acidovorax citrulli ... See more keywords