A Comparative Reengineering Study of cpADH5 through Iterative and Simultaneous Multisite Saturation Mutagenesis
Sign Up to like & getrecommendations! Published in 2018 at "ChemBioChem"
DOI: 10.1002/cbic.201800159
Abstract: Positions identified in directed evolution campaigns or by (semi)rational design can be recombined iteratively or simultaneously. Iterative recombination has yielded many success stories and is beneficially used if screening capabilities are limited (four iterative SSMs… read more here.
Keywords: saturation mutagenesis; simultaneous multisite; iterative simultaneous; saturation ... See more keywords
Multiple Site-Directed and Saturation Mutagenesis by the Patch Cloning Method.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6472-7_22
Abstract: Constructing protein-coding genes with desired mutations is a basic step for protein engineering. Herein, we describe a multiple site-directed and saturation mutagenesis method, termed MUPAC. This method has been used to introduce multiple site-directed mutations… read more here.
Keywords: saturation mutagenesis; multiple site; directed saturation; site directed ... See more keywords
MutateX: an automated pipeline for in silico saturation mutagenesis of protein structures and structural ensembles
Sign Up to like & getrecommendations! Published in 2022 at "Briefings in bioinformatics"
DOI: 10.1093/bib/bbac074
Abstract: Mutations, which result in amino acid substitutions, influence the stability of proteins and their binding to biomolecules. A molecular understanding of the effects of protein mutations is both of biotechnological and medical relevance. Empirical free… read more here.
Keywords: automated pipeline; saturation mutagenesis; silico saturation; protein ... See more keywords
fastISM: Performant in-silico saturation mutagenesis for convolutional neural networks.
Sign Up to like & getrecommendations! Published in 2022 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btac135
Abstract: MOTIVATION Deep learning models such as convolutional neural networks are able to accurately map biological sequences to associated functional readouts and properties by learning predictive de novo representations. In-silico saturation mutagenesis (ISM) is a popular… read more here.
Keywords: saturation mutagenesis; neural networks; fastism; convolutional neural ... See more keywords
Highly efficient libraries design for saturation mutagenesis
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DOI: 10.1093/synbio/ysac006
Abstract: Abstract Saturation mutagenesis is a semi-rational approach for protein engineering where sites are saturated either entirely or partially to include amino acids of interest. We previously reported on a codon compression algorithm, where a set… read more here.
Keywords: saturation; highly efficient; libraries design; efficient libraries ... See more keywords
A saturation-mutagenesis analysis of the interplay between stability and activation in Ras
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DOI: 10.1101/2022.01.06.475190
Abstract: Cancer mutations in Ras occur predominantly at three hotspots: Gly 12, Gly 13, and Gln 61. Previously, we reported that deep mutagenesis of H-Ras using a bacterial assay identified many other activating mutations (Bandaru et… read more here.
Keywords: activation; cancer hotspots; stability; cancer ... See more keywords
Computational Saturation Mutagenesis to Investigate the Effects of Neurexin-1 Mutations on AlphaFold Structure
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DOI: 10.3390/genes13050789
Abstract: Neurexin-1 (NRXN1) is a membrane protein essential in synapse formation and cell signaling as a cell-adhesion molecule and cell-surface receptor. NRXN1 and its binding partner neuroligin have been associated with deficits in cognition. Recent genetics… read more here.
Keywords: structure; mutations alphafold; alphafold structure; computational saturation ... See more keywords