Genome-scale CRISPR pooled screens.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2016.05.014
Abstract: Genome editing technologies such as clustered regularly interspaced short palindromic repeats (CRISPR) systems have ushered in a new era of targeted DNA manipulation. The easy programmability of CRISPR using short oligonucleotides enables rapid synthesis of… read more here.
Keywords: biochemistry; genome scale; crispr; pooled screens ... See more keywords
A genome-scale CRISPR/Cas9 knockout screening reveals SH3D21 as a sensitizer for gemcitabine
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-55893-2
Abstract: Gemcitabine, 2′,2′-difluoro-2′-deoxycytidine, is used as a pro-drug in treatment of variety of solid tumour cancers including pancreatic cancer. After intake, gemcitabine is transferred to the cells by the membrane nucleoside transporter proteins. Once inside the… read more here.
Keywords: genome scale; gemcitabine; crispr cas9; scale crispr ... See more keywords
Abstract 257: CDX model based genome-scale CRISPR KO screens for modulators of KRAS (G12C) inhibitor sensitivity
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Research"
DOI: 10.1158/1538-7445.am2023-257
Abstract: KRAS is the most frequently mutated oncogene in human cancers and had long been considered an undruggable target for cancer therapy, until the FDA approval of Sotorasib (AMG 510) for the treatment of patients with… read more here.
Keywords: cell; kras g12c; scale crispr; cancer ... See more keywords
Genome-scale CRISPR screening at high sensitivity with an empirically designed sgRNA library
Sign Up to like & getrecommendations! Published in 2020 at "BMC Biology"
DOI: 10.1186/s12915-020-00905-1
Abstract: Background In recent years, large-scale genetic screens using the CRISPR/Cas9 system have emerged as scalable approaches able to interrogate gene function with unprecedented efficiency and specificity in various biological contexts. By this means, functional dependencies… read more here.
Keywords: genome scale; crispr; sgrna library; empirically designed ... See more keywords
Genome-scale CRISPR screening for modifiers of cellular LDL uptake.
Sign Up to like & getrecommendations! Published in 2021 at "PLoS genetics"
DOI: 10.1371/journal.pgen.1009285
Abstract: Hypercholesterolemia is a causal and modifiable risk factor for atherosclerotic cardiovascular disease. A critical pathway regulating cholesterol homeostasis involves the receptor-mediated endocytosis of low-density lipoproteins into hepatocytes, mediated by the LDL receptor. We applied genome-scale… read more here.
Keywords: genome scale; ldl uptake; scale crispr; cellular ldl ... See more keywords
Genome-scale CRISPR screening reveals that C3aR signaling is critical for rapid capture of fungi by macrophages
Sign Up to like & getrecommendations! Published in 2022 at "PLoS Pathogens"
DOI: 10.1371/journal.ppat.1010237
Abstract: The fungal pathogen Histoplasma capsulatum (Hc) invades, replicates within, and destroys macrophages. To interrogate the molecular mechanisms underlying this interaction, we conducted a host-directed CRISPR-Cas9 screen and identified 361 genes that modify macrophage susceptibility to… read more here.
Keywords: scale crispr; fungi; capture; c3ar ... See more keywords
A Genome-Scale CRISPR Knock-Out Screen Identifies MicroRNA-5197-5p as a Promising Radiosensitive Biomarker in Colorectal Cancer
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Oncology"
DOI: 10.3389/fonc.2021.696713
Abstract: Radioresistance is one of the main reasons causing unsatisfactory curative effects of ionizing radiation (IR) against colorectal cancer (CRC). However, its underlying mechanisms remain unclear yet. In the present study, we applied a genome-scale CRISPR… read more here.
Keywords: microrna 5197; genome scale; colorectal cancer; scale crispr ... See more keywords