Thermal Shift and Stability Assays of Disease-Related Misfolded Proteins Using Differential Scanning Fluorimetry.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8820-4_16
Abstract: Systematic identification of buffer formulations and small molecule chaperones that improve the expression, stability, and storage of proteins with therapeutic interest has gained enormous importance in biochemical research as well as in biotechnology and biomedical… read more here.
Keywords: thermal shift; differential scanning; scanning fluorimetry; stability ... See more keywords
Differential scanning fluorimetry followed by microscale thermophoresis and/or isothermal titration calorimetry as an efficient tool for ligand screening
Sign Up to like & getrecommendations! Published in 2025 at "Biophysical Reviews"
DOI: 10.1007/s12551-025-01280-3
Abstract: Various biophysical and biochemical techniques have been developed to measure the affinity of interacting molecules. This review analyzes the combination of three methods: differential scanning fluorimetry as the initial high-throughput screening technique and microscale thermophoresis… read more here.
Keywords: thermophoresis isothermal; isothermal titration; titration calorimetry; microscale thermophoresis ... See more keywords
In-situ and sensitive stability study of emulsion and aluminum adjuvanted inactivated foot-and-mouth disease virus vaccine by differential scanning fluorimetry analysis.
Sign Up to like & getrecommendations! Published in 2020 at "Vaccine"
DOI: 10.1016/j.vaccine.2020.02.068
Abstract: Adjuvants are important to enhance the antigens immunogenicity, but may also alter the structures of antigens. Currently off-line methods for adjuvants induced antigen alteration suffer from incomplete release and possible structural alteration of antigens. Here… read more here.
Keywords: differential scanning; foot mouth; inactivated foot; scanning fluorimetry ... See more keywords
Intrinsic Differential Scanning Fluorimetry for Protein Stability Assessment in Microwell Plates
Sign Up to like & getrecommendations! Published in 2025 at "Molecular Pharmaceutics"
DOI: 10.1021/acs.molpharmaceut.4c01496
Abstract: Intrinsic differential scanning fluorimetry (DSF) is essential for analyzing protein thermal stability. Until now, intrinsic DSF was characterized by medium throughput and high consumable costs. Here, we present a microplate-based intrinsic DSF approach that enables… read more here.
Keywords: intrinsic dsf; stability; protein; differential scanning ... See more keywords
Screening of benzenesulfonamide in combination with chemically diverse fragments against carbonic anhydrase by differential scanning fluorimetry
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Enzyme Inhibition and Medicinal Chemistry"
DOI: 10.1080/14756366.2019.1698562
Abstract: Abstract The differential scanning fluorimetry (DSF) screening of 5.692 fragments in combination with benzenesulfonamide (BSA) against bovine carbonic anhydrase (bCA) delivered >100 hits that either caused, on their own, a significant thermal shift (ΔTm ,… read more here.
Keywords: combination; carbonic anhydrase; differential scanning; screening benzenesulfonamide ... See more keywords
Combining nano-differential scanning fluorimetry and microscale thermophoresis to investigate VDAC1 interaction with small molecules
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Enzyme Inhibition and Medicinal Chemistry"
DOI: 10.1080/14756366.2022.2121821
Abstract: Abstract The mitochondrial voltage-dependent anion channel 1 (VDAC1) plays a central role in metabolism and apoptosis, which makes it a promising therapeutic target. Nevertheless, molecular mechanisms governing VDAC1 functioning remain unclear. Small-molecule ligands specifically interacting… read more here.
Keywords: vdac1; microscale thermophoresis; fluorimetry microscale; differential scanning ... See more keywords
Application of temperature-responsive HIS-tag fluorophores to differential scanning fluorimetry screening of small molecule libraries
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Pharmacology"
DOI: 10.3389/fphar.2022.1040039
Abstract: Differential scanning fluorimetry is a rapid and economical biophysical technique used to monitor perturbations to protein structure during a thermal gradient most often by detecting protein unfolding events through an environment-sensitive fluorophore. By employing an… read more here.
Keywords: dsf assay; temperature; differential scanning; scanning fluorimetry ... See more keywords
Discovery of Novel Inhibitors against ALS-Related SOD1(A4V) Aggregation through the Screening of a Chemical Library Using Differential Scanning Fluorimetry (DSF)
Sign Up to like & getrecommendations! Published in 2024 at "Pharmaceuticals"
DOI: 10.3390/ph17101286
Abstract: Background: Cu/Zn Superoxide Dismutase 1 (SOD1) is a 32 kDa cytosolic dimeric metalloenzyme that neutralizes superoxide anions into oxygen and hydrogen peroxide. Mutations in SOD1 are associated with ALS, a disease causing motor neuron atrophy… read more here.
Keywords: using differential; sod1 a4v; differential scanning; aggregation ... See more keywords
Nano Differential Scanning Fluorimetry as a Rapid Stability Assessment Tool in the Nanoformulation of Proteins
Sign Up to like & getrecommendations! Published in 2023 at "Pharmaceutics"
DOI: 10.3390/pharmaceutics15051473
Abstract: The development and production of innovative protein-based therapeutics is a complex and challenging avenue. External conditions such as buffers, solvents, pH, salts, polymers, surfactants, and nanoparticles may affect the stability and integrity of proteins during… read more here.
Keywords: differential scanning; stability; scanning fluorimetry; nano differential ... See more keywords