Isolation and characterization of a novel scFv antibody fragments specific for Hsp70 as a tumor biomarker
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Cellular Biochemistry"
DOI: 10.1002/jcb.28732
Abstract: Many studies have shown that more than 50% of tumors express heat shock protein 70 kDa (Hsp70) at the plasma membrane surface while not seen in normal cells, therefore it is a promising therapeutic target… read more here.
Keywords: scfv; isolation characterization; human hsp70; scfv antibody ... See more keywords
Editing of DNA Methylation Using dCas9-Peptide Repeat and scFv-TET1 Catalytic Domain Fusions.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7774-1_23
Abstract: DNA methylation, one of the most studied epigenetic modifications, regulates many biological processes. Dysregulation of DNA methylation is implicated in the etiology of several diseases, such as cancer and imprinting diseases. Accordingly, technologies designed to… read more here.
Keywords: methylation using; editing dna; dna; scfv ... See more keywords
Development of humanized scFv antibody fragment(s) that targets and blocks specific HLA alleles linked to myasthenia gravis
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8557-1
Abstract: Myasthenia gravis (MG) is an autoimmune disease caused by sensitization of the immune system to self-antigens. We have previously shown that targeting MG-susceptible alleles can significantly inhibit proliferation of disease-specific T cells. In this work,… read more here.
Keywords: humanized scfv; antibody; hla; scfv ... See more keywords
Construction and characterization of a class-specific single-chain variable fragment against pyrethroid metabolites
Sign Up to like & getrecommendations! Published in 2020 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-020-10728-3
Abstract: Pyrethroids are insecticides that are widely used in rural and urban areas worldwide. After entering the environment, pyrethroids are rapidly metabolized or degraded by various biological or abiotic methods. In this study, a single-chain variable… read more here.
Keywords: pyrethroid metabolites; scfv; chain variable; variable fragment ... See more keywords
An optimized yeast display strategy for efficient scFv antibody selection using ribosomal skipping system and thermo resistant yeast
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology Letters"
DOI: 10.1007/s10529-019-02710-5
Abstract: ObjectivesEstablish a method to restrict unexpected fragments including stop codons in scFv library and generate a thermo resistant strain for screening of thermal stable scFv sequences.ResultsHere, we have constructed a T2A–Leu2 system for selection of… read more here.
Keywords: ribosomal skipping; thermo resistant; resistant yeast; selection ... See more keywords
Expression of Single Chain Variable Fragment (scFv) Molecules in Plants: A Comprehensive Update
Sign Up to like & getrecommendations! Published in 2020 at "Molecular Biotechnology"
DOI: 10.1007/s12033-020-00241-3
Abstract: Single chain variable fragments (scFvs) are generated by joining together the variable heavy and light chain of a monoclonal antibody (mAb) via a peptide linker. They offer some advantages over the parental mAb such as… read more here.
Keywords: chain; expression single; scfv; expression ... See more keywords
Virtual mutation and directional evolution of anti-amoxicillin ScFv antibody for immunoassay of penicillins in milk.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2016.10.020
Abstract: In this study, an anti-amoxicillin single chain variable fragment (ScFv) antibody was evolved by directional mutagenesis of a contact amino acid residue based on the analysis of virtual mutation. Comparison with its parental ScFv, the… read more here.
Keywords: anti amoxicillin; antibody; scfv; virtual mutation ... See more keywords
An E. coli‐produced single‐chain variable fragment (scFv) targeting hepatitis B virus surface protein potently inhibited virion secretion
Sign Up to like & getrecommendations! Published in 2019 at "Antiviral Research"
DOI: 10.1016/j.antiviral.2018.12.019
Abstract: ABSTRACT Hepatitis B virus (HBV) envelopes as well as empty subviral particles carry in their lipid membranes the small (S), middle (M), and large (L) surface proteins, collectively known as hepatitis B surface antigen (HBsAg).… read more here.
Keywords: virion; g12 scfv; secretion; hepatitis ... See more keywords
99mTc(CO)3+ labeled domain I/II-specific anti-EGFR (scFv)2 antibody fragment for imaging EGFR expression.
Sign Up to like & getrecommendations! Published in 2018 at "European journal of medicinal chemistry"
DOI: 10.1016/j.ejmech.2018.08.014
Abstract: Bifunctional chelators (BFCs) are covalently linked to biologically active targeting molecules and radiolabeled with radiometals. Technetium-99 m (99mTc) is the most widely used isotope in nuclear medicine because of its excellent physical properties. The objective of… read more here.
Keywords: antibody; scfv; anti egfr; dlo6 scfv ... See more keywords
Preparation and in vitro activity of single chain antibodies against Alzheimer's disease.
Sign Up to like & getrecommendations! Published in 2020 at "Immunology letters"
DOI: 10.1016/j.imlet.2020.07.009
Abstract: Disease-modifying passive immunotherapies focused on reducing amyloid-beta (Aβ) deposition are a potential therapeutic strategy for Alzheimer's disease (AD). However, the results of Aβ passive immunotherapy clinical trials were unsatisfactory, largely due to the low efficacy… read more here.
Keywords: alzheimer disease; disease; scfv; activity ... See more keywords
Characterization of a Novel Bispecific Antibody with Improved Conformational and Chemical Stability.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of pharmaceutical sciences"
DOI: 10.1016/j.xphs.2019.06.025
Abstract: Bispecific antibodies containing single chain variable fragment (scFv) appended to IgGs offer unique development challenges. Here we describe the stability of a novel bispecific format, BiS5, where the scFv is tethered to the CH3 domain.… read more here.
Keywords: scfv; stability; novel bispecific; chemical stability ... See more keywords