Isolation and characterization of a novel scFv antibody fragments specific for Hsp70 as a tumor biomarker
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Cellular Biochemistry"
DOI: 10.1002/jcb.28732
Abstract: Many studies have shown that more than 50% of tumors express heat shock protein 70 kDa (Hsp70) at the plasma membrane surface while not seen in normal cells, therefore it is a promising therapeutic target… read more here.
Keywords: scfv; isolation characterization; human hsp70; scfv antibody ... See more keywords
Virtual mutation and directional evolution of anti-amoxicillin ScFv antibody for immunoassay of penicillins in milk.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2016.10.020
Abstract: In this study, an anti-amoxicillin single chain variable fragment (ScFv) antibody was evolved by directional mutagenesis of a contact amino acid residue based on the analysis of virtual mutation. Comparison with its parental ScFv, the… read more here.
Keywords: anti amoxicillin; antibody; scfv; virtual mutation ... See more keywords
Accumulative scFv-Fc antibody gene integration into the hprt chromosomal locus of Chinese hamster ovary cells.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of bioscience and bioengineering"
DOI: 10.1016/j.jbiosc.2017.05.017
Abstract: We have previously developed an accumulative site-specific gene integration system (AGIS) using Cre-recombinase and mutated loxP sites. AGIS enables repeated transgene integration into a predetermined chromosomal site in mammalian cells. However, the process of establishing… read more here.
Keywords: integration; hamster ovary; chinese hamster; gene integration ... See more keywords