CircRNA expression profile of bovine placentas in late gestation with aberrant SCNT fetus
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.22918
Abstract: One of the limitations of somatic cell nuclear transfer (SCNT) strategy to generate genetically modified offspring is the low birth rate. Placental dysfunction is one of the causes of abortion. Circular RNA (circRNA) is noncoding… read more here.
Keywords: expression profile; profile bovine; bovine placentas; circrna ... See more keywords
Sperm‐derived RNAs improve the efficiency of somatic cell nuclear transfer (SCNT) through promoting R‐loop formation
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Reproduction and Development"
DOI: 10.1002/mrd.23627
Abstract: Mammalian sperm and oocytes are haploid cells that carry parental genetic and epigenetic information for their progeny. The cytoplasm of oocytes is also capable of reprograming somatic cells to establish totipotency through somatic cell nuclear… read more here.
Keywords: sperm derived; somatic cell; cell nuclear; sperm ... See more keywords
Production of cloned cats using additional complimentary cytoplasm.
Sign Up to like & getrecommendations! Published in 2019 at "Animal reproduction science"
DOI: 10.1016/j.anireprosci.2019.106125
Abstract: Somatic cell nuclear transfer (SCNT) is an important technique for producing cloned animals. It, however, is inefficient when there is use of SCNT for cloned animal production. Cytoplasm injection cloning technology (CICT) was developed to… read more here.
Keywords: production; cict; cloned embryos; cytoplasm ... See more keywords
RS-1 enhanced the efficiency of somatic cell reprogramming via activation of RAD51 in somatic cell nuclear transfer
Sign Up to like & getrecommendations! Published in 2020 at "Cytotherapy"
DOI: 10.1016/j.jcyt.2020.03.208
Abstract: Background & Aim Reprogramming efficiency of somatic cell nuclei is very low in both somatic cell nuclear transfer (SCNT) and iPSCs technologies. Recently, it was reported that the genetic instability and DNA damage arise prior… read more here.
Keywords: somatic cell; group; expression; efficiency ... See more keywords
Anti-apoptotic Regulation Contributes to the Successful Nuclear Reprogramming Using Cryopreserved Oocytes
Sign Up to like & getrecommendations! Published in 2019 at "Stem Cell Reports"
DOI: 10.1016/j.stemcr.2019.01.019
Abstract: Summary Cryopreservation has a negative effect on the quality of oocytes and may be closely associated with increased levels of reactive oxygen species (ROS) and apoptotic events. The purpose of the present study was to… read more here.
Keywords: embryos using; apoptotic regulation; anti apoptotic; using cryopreserved ... See more keywords
Transcriptome-wide analysis of the SCNT bovine abnormal placenta during mid- to late gestation
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-56566-w
Abstract: The dysfunction of placenta is common in somatic cell nuclear transfer (SCNT) cloned cattle and would cause aberrant fetal development and even abortion, which occurred with highest rate at the mid- to late gestation. However,… read more here.
Keywords: gestation; scnt; placenta; transcriptome wide ... See more keywords
19 Improvement of the developmental competence of bovine somatic cell nuclear transfer embryos using latrunculin A during activation
Sign Up to like & getrecommendations! Published in 2020 at "Reproduction, Fertility and Development"
DOI: 10.1071/rdv32n2ab19
Abstract: Somatic cell nuclear transfer (SCNT) is an assisted reproductive technology with potential for its application in agriculture, biomedicine, and biotechnology. However, the SCNT efficiency is low. Failure in embryo production by SCNT could be associated… read more here.
Keywords: part; lata; scnt demec; ctrol ... See more keywords
Improved development of mouse somatic cell nuclear transfer embryos by chlamydocin analogues, class I and IIa histone deacetylase inhibitors†
Sign Up to like & getrecommendations! Published in 2021 at "Biology of Reproduction"
DOI: 10.1093/biolre/ioab096
Abstract: Abstract In mammalian cloning by somatic cell nuclear transfer (SCNT), the treatment of reconstructed embryos with histone deacetylase (HDAC) inhibitors improves efficiency. So far, most of those used for SCNT are hydroxamic acid derivatives—such as… read more here.
Keywords: class; chlamydocin analogues; class iia; scnt ... See more keywords
Down-Regulation of H3K4me3 by MM-102 Facilitates Epigenetic Reprogramming of Porcine Somatic Cell Nuclear Transfer Embryos
Sign Up to like & getrecommendations! Published in 2018 at "Cellular Physiology and Biochemistry"
DOI: 10.1159/000487579
Abstract: Background/Aims: Aberrantly high levels of H3K4me3, caused by incomplete epigenetic reprogramming, likely cause a low efficiency of somatic cell nuclear transfer (SCNT). Smal molecule inhibitors aimed at epigenetic modification can be used to improve porcine… read more here.
Keywords: somatic cell; cell nuclear; embryos; nuclear transfer ... See more keywords
Choosing a culture medium for SCNT and iSCNT reconstructed embryos: from domestic to wildlife species
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Animal Science and Technology"
DOI: 10.1186/s40781-017-0149-1
Abstract: Over the past decades, in vitro culture media have been developed to successfully support IVF embryo growth in a variety of species. Advanced reproductive technologies, such as somatic cell nuclear transfer (SCNT), challenge us with… read more here.
Keywords: embryos domestic; scnt; wildlife species; culture ... See more keywords
Melatonin accelerates the developmental competence and telomere elongation in ovine SCNT embryos
Sign Up to like & getrecommendations! Published in 2022 at "PLoS ONE"
DOI: 10.1371/journal.pone.0267598
Abstract: SCNT embryos suffer from poor developmental competence (both in vitro and in vivo) due to various defects such as oxidative stress, incomplete epigenetic reprogramming, and flaws in telomere rejuvenation. It is very promising to ameliorate… read more here.
Keywords: scnt embryos; ovine scnt; melatonin; scnt ... See more keywords