A novel approach for scrapie-associated prion (PrPSc) detection in blood using the competitive affinity of an aggregate-specific antibody and streptavidin to PrPSc.
Sign Up to like & getrecommendations! Published in 2017 at "Research in veterinary science"
DOI: 10.1016/j.rvsc.2017.09.007
Abstract: Scrapie is a fatal neurodegenerative disorder affecting sheep and goats, originating from exposure to disease-associated prions (PrPSc). An ante-mortem screening test that can detect native PrPSc in body fluids remains unavailable due to insufficient sensitivity… read more here.
Keywords: detection; blood using; antibody; scrapie ... See more keywords
A decade of using small-to-medium throughput allele discrimination assay to determine prion protein gene (Prnp) genotypes in sheep in Slovenia
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Veterinary Diagnostic Investigation"
DOI: 10.1177/1040638717723946
Abstract: Sheep with valine (V) at codon 136 and glutamine (Q) at codon 171 of the prion protein gene (Prnp) are highly susceptible to classical scrapie, whereas phenylalanine (F) at codon 141 and histidine (H) at… read more here.
Keywords: small medium; scrapie; classical scrapie; prion protein ... See more keywords
Second passage of chronic wasting disease of mule deer to sheep by intracranial inoculation compared to classical scrapie
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Veterinary Diagnostic Investigation"
DOI: 10.1177/10406387211017615
Abstract: The origin of chronic wasting disease (CWD) in cervids is unclear. One hypothesis suggests that CWD originated from scrapie in sheep. We compared the disease phenotype of sheep-adapted CWD to classical scrapie in sheep. We… read more here.
Keywords: cwd; scrapie; disease; classical scrapie ... See more keywords
Autoclave treatment of the classical scrapie agent US No. 13-7 and experimental inoculation to susceptible VRQ/ARQ sheep via the oral route results in decreased transmission efficiency
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DOI: 10.1371/journal.pone.0243009
Abstract: Scrapie, a prion disease of sheep, is highly resistant to conventional deactivation. Numerous methods to deactivate scrapie have been tested in laboratory animal models, and adequate autoclave treatment can reduce or remove the infectivity of… read more here.
Keywords: susceptible vrq; autoclave treatment; scrapie; classical scrapie ... See more keywords
PrPres in placental tissue following experimental transmission of atypical scrapie in ARR/ARR sheep is not infectious by Tg338 mouse bioassay
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DOI: 10.1371/journal.pone.0262766
Abstract: Nor98-like atypical scrapie is a sporadic disease that affects the central nervous system of sheep and goats that, in contrast to classical scrapie, is not generally regarded as naturally transmissible. However, infectivity has been demonstrated… read more here.
Keywords: scrapie; nor98 like; arr arr; arr ... See more keywords
Combinatorial treatment of brain samples from sheep with scrapie using sodium percarbonate, sodium dodecyl sulfate, and proteinase K increases survival time in inoculated susceptible sheep.
Sign Up to like & getrecommendations! Published in 2022 at "Research in veterinary science"
DOI: 10.2139/ssrn.4134193
Abstract: The agent of scrapie is resistant to most chemical and physical methods of inactivation. Prions bind to soils, metals, and various materials and persist in the environment confounding the control of prion diseases. Most methods… read more here.
Keywords: sodium dodecyl; months survival; scrapie; brain ... See more keywords
The Scrapie Prevalence in a Goat Herd Is Underestimated by Using a Rapid Diagnostic Test
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2020.00164
Abstract: Current European surveillance regulations for scrapie, a naturally occurring transmissible spongiform encephalopathy (TSE) or prion disease in sheep and goats, require testing of fallen stock or healthy slaughter animals, and outline measures in the case… read more here.
Keywords: scrapie; confirmatory tests; prion protein; test ... See more keywords
Evaluation of Antemortem Diagnostic Techniques in Goats Naturally Infected With Scrapie
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DOI: 10.3389/fvets.2020.517862
Abstract: Scrapie is a naturally occurring transmissible spongiform encephalopathy (TSE) that affects sheep and goats. Sheep and goats can be infected with scrapie as lambs or kids via contact with the placenta or placental fluids, or… read more here.
Keywords: scrapie; period; antemortem; naturally infected ... See more keywords
Genome-Wide Methylation Profiling in the Thalamus of Scrapie Sheep
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Veterinary Science"
DOI: 10.3389/fvets.2022.824677
Abstract: Scrapie is a neurodegenerative disorder belonging to the group of transmissible spongiform encephalopathy (TSE). Scrapie occurs in sheep and goats, which are considered good natural animal models of these TSE. Changes in DNA methylation occur… read more here.
Keywords: methylation; dna methylation; scrapie; genome wide ... See more keywords
Prediction of Genetic Resistance for Scrapie in Ungenotyped Sheep Using a Linear Animal Model
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DOI: 10.3390/genes12091432
Abstract: Selection based on scrapie genotypes could improve the genetic resistance for scrapie in sheep. However, in practice, few animals are genotyped. The objectives were to define numerical values of scrapie resistance genotypes and adjust for… read more here.
Keywords: animal model; linear animal; scrapie; resistance ... See more keywords
5-Methylcytosine and 5-Hydroxymethylcytosine in Scrapie-Infected Sheep and Mouse Brain Tissues
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24021621
Abstract: Scrapie is a neurodegenerative disorder belonging to the group of transmissible spongiform encephalopathies or prion diseases, which are caused by an infectious isoform of the innocuous cellular prion protein (PrPC) known as PrPSc. DNA methylation,… read more here.
Keywords: prion; scrapie; sheep mouse; scrapie infected ... See more keywords