Hybridoma Screening by Antibody Capture: Flow Cytometry/FACS with Permeabilized Cells to Detect Intracellular Binding.
Sign Up to like & getrecommendations! Published in 2022 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot103085
Abstract: Flow cytometry or fluorescence-activated cell sorting (FACS) can be used to identify hybridomas secreting monoclonal antibodies to internal cellular proteins, but the cells must be permeabilized before the hybridoma supernatants are applied. In using this… read more here.
Keywords: hybridoma screening; flow cytometry; screening antibody; cytometry ... See more keywords
Hybridoma Screening by Antibody Capture: Detection of Cell-Surface Binding by Immunofluorescence.
Sign Up to like & getrecommendations! Published in 2022 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot103093
Abstract: If the antigen of interest is a cell-surface protein, immunofluorescence can be used to identify hybridomas secreting monoclonal antibodies to these proteins. They can be stained on microscope chamber slides, flat-bottomed cell culture plates (96,… read more here.
Keywords: cell; hybridoma screening; cell surface; screening antibody ... See more keywords
Hybridoma Screening by Antibody Capture: Detection of Intracellular Binding by Immunofluorescence.
Sign Up to like & getrecommendations! Published in 2022 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot103101
Abstract: Hybridoma screening by immunofluorescence stainings of whole cells can be adapted to screen for antibodies to internal proteins by permeabilizing the cells before applying the hybridoma supernatants. In this protocol, cells are attached to a… read more here.
Keywords: capture detection; hybridoma screening; screening antibody; immunofluorescence ... See more keywords
Single-step colony assay with autoinduction of scFv expression for the screening of antibody libraries.
Sign Up to like & getrecommendations! Published in 2019 at "BioTechniques"
DOI: 10.2144/btn-2018-0195
Abstract: We present a simple colony assay method for screening antibody libraries based on autoinduction of antibody fragment expression. This protocol eliminates the need for colony size monitoring and a separate induction step for single-chain Fv… read more here.
Keywords: antibody libraries; antibody; colony assay; expression ... See more keywords