Nutrient Stress Small-Molecule Screening Platform for Escherichia coli.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7847-2_1
Abstract: Clinically approved antibiotics target a narrow spectrum of cellular processes, namely cell wall synthesis, DNA replication, and protein synthesis. Numerous screens have been designed to identify inhibitors that target one of these cellular processes. Indeed,… read more here.
Keywords: escherichia coli; coli nutrient; nutrient stress; stress ... See more keywords
Imaging-Based Screening Platform Assists Protein Engineering.
Sign Up to like & getrecommendations! Published in 2018 at "Cell chemical biology"
DOI: 10.1016/j.chembiol.2018.08.008
Abstract: Protein engineering involves generating and screening large numbers of variants for desired properties. While modern DNA technology has made it easy to create protein diversity on the DNA level, the selection and validation of candidate… read more here.
Keywords: protein engineering; platform; screening platform; based screening ... See more keywords
Modeling the mature CNS: A predictive screening platform for neurodegenerative disease drug discovery
Sign Up to like & getrecommendations! Published in 2021 at "Journal of neuroscience methods"
DOI: 10.1016/j.jneumeth.2021.109180
Abstract: Background: Mature primary neuronal cultures are an important model of the nervous system, but limited scalability has been a major challenge in their use for drug discovery of neurodegenerative diseases. This work describes a method… read more here.
Keywords: mature; screening platform; disease; drug discovery ... See more keywords
Ex Vivo Cell-Based Screening Platform for Modulators of Hepatosteatosis.
Sign Up to like & getrecommendations! Published in 2017 at "ACS chemical biology"
DOI: 10.1021/acschembio.7b00420
Abstract: Nonalcoholic fatty liver disease (NAFLD) is the result of the ectopic accumulation of lipids in hepatic cells and is the early stage of liver diseases including fibrosis, cirrhosis, and hepatocellular carcinoma. While some mechanisms of… read more here.
Keywords: cell based; vivo cell; platform; screening platform ... See more keywords
A logical network-based drug-screening platform for Alzheimer’s disease representing pathological features of human brain organoids
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-020-20440-5
Abstract: Developing effective drugs for Alzheimer’s disease (AD), the most common cause of dementia, has been difficult because of complicated pathogenesis. Here, we report an efficient, network-based drug-screening platform developed by integrating mathematical modeling and the… read more here.
Keywords: network based; alzheimer disease; pathological features; drug screening ... See more keywords
High-Throughput Screening Platform To Identify Inhibitors of Protein Synthesis with Potential for the Treatment of Malaria
Sign Up to like & getrecommendations! Published in 2022 at "Antimicrobial Agents and Chemotherapy"
DOI: 10.1128/aac.00237-22
Abstract: Artemisinin-based combination therapies have been crucial in driving down the global burden of malaria, the world’s largest parasitic killer. However, their efficacy is now threatened by the emergence of resistance in Southeast Asia and sub-Saharan… read more here.
Keywords: inhibitors protein; platform; screening platform; throughput screening ... See more keywords
Development of a High-throughput NanoBRET Screening Platform to Identify Modulators of the RAS/RAF Interaction
Sign Up to like & getrecommendations! Published in 2021 at "Molecular Cancer Therapeutics"
DOI: 10.1158/1535-7163.mct-21-0175
Abstract: Activating mutations in RAS are found in approximately 30% of human cancers, resulting in the delivery of a persistent signal to critical downstream effectors that drive tumorigenesis. RAS-driven malignancies respond poorly to conventional cancer treatments… read more here.
Keywords: screening platform; platform identify; development; ras raf ... See more keywords
Abstract 506: A bespoke screening platform to study mono(ADP-ribosylation)
Sign Up to like & getrecommendations! Published in 2020 at "Cancer Research"
DOI: 10.1158/1538-7445.am2020-506
Abstract: Mono(ADP-ribosylation) (MARylation) and poly(ADP-ribosylation) (PARylation) are post-translational modifications deposited on multiple amino acids by PARP enzymes using nicotinamide adenine dinucleotide (NAD+) as the ADP-ribose donating substrate. While there are approved drugs and clinical trials on-going… read more here.
Keywords: adp ribosylation; screening platform; bespoke screening; mono adp ... See more keywords
Abstract 762: Organo-on-pillar based anticancer drug screening platform for ovarian cancer
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Research"
DOI: 10.1158/1538-7445.am2023-762
Abstract: Previously preclinical models for customized drug screening and precision oncology are being developed to replace animal models. Advances in biocompatible organoid technology have made it possible to culture cancer organoids from human tumor tissues. Cancer… read more here.
Keywords: drug screening; anticancer drug; screening platform; drug ... See more keywords
A clinically compatible drug‐screening platform based on organotypic cultures identifies vulnerabilities to prevent and treat brain metastasis
Sign Up to like & getrecommendations! Published in 2022 at "EMBO Molecular Medicine"
DOI: 10.15252/emmm.202114552
Abstract: We report a medium‐throughput drug‐screening platform (METPlatform) based on organotypic cultures that allows to evaluate inhibitors against metastases growing in situ. By applying this approach to the unmet clinical need of brain metastasis, we identified… read more here.
Keywords: metastasis; drug screening; screening platform; brain ... See more keywords
A Human TSC1 Variant Screening Platform in Gabaergic Cortical Interneurons for Genotype to Phenotype Assessments
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Molecular Neuroscience"
DOI: 10.3389/fnmol.2020.573409
Abstract: The TSC1 and TSC2 genes are connected to multiple syndromes from Tuberous Sclerosis Complex (TSC) to autism spectrum disorder (ASD), with uncertainty if genetic variants cause all or subsets of phenotypes based on the location… read more here.
Keywords: tsc1; genotype phenotype; human tsc1; variant screening ... See more keywords