Phenotypic screening with target identification and validation in the discovery and development of E3 ligase modulators.
Sign Up to like & getrecommendations! Published in 2021 at "Cell chemical biology"
DOI: 10.1016/j.chembiol.2021.02.011
Abstract: The use of phenotypic screening was central to the discovery and development of novel thalidomide analogs, the IMiDs (immunomodulatory drugs) agents. With the discovery that these agents bind the E3 ligase, CRL4CRBN, and alter its… read more here.
Keywords: discovery development; phenotypic screening; discovery; screening target ... See more keywords
Proteomics-based screening of the target proteins associated with antidepressant-like effect and mechanism of nimesulide
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-66420-z
Abstract: Nimesulide is an inhibitor of COX-2 with antioxidant and anti-inflammatory effects. However, few studies have explored the antidepressant mechanism of nimesulide. Here, we evaluated the therapeutic effects of nimesulide on CUMS rats. iTRAQ technology was… read more here.
Keywords: mechanism; effect; proteomics based; screening target ... See more keywords