Purification of recombinant Aβ(1‐42) and pGlu‐Aβ(3‐42) using preparative SDS‐PAGE
Sign Up to like & getrecommendations! Published in 2017 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201700154
Abstract: Recombinant expression and purification of amyloid peptides represents a common basis for investigating the molecular mechanisms of amyloid formation and toxicity. However, the isolation of the recombinant peptides is hampered by inefficient separation from contaminants… read more here.
Keywords: sds page; purification; recombinant; preparative sds ... See more keywords
A comparative study of CE‐SDS, SDS‐PAGE, and Simple Western—Precision, repeatability, and apparent molecular mass shifts by glycosylation
Sign Up to like & getrecommendations! Published in 2021 at "ELECTROPHORESIS"
DOI: 10.1002/elps.202100068
Abstract: SDS gel electrophoresis is a commonly used approach for monitoring purity and apparent molecular mass (Mr) of proteins, especially in the field of quality control of biopharmaceutical proteins. The technological installation of CE‐SDS as the… read more here.
Keywords: molecular mass; sds sds; sds; sds page ... See more keywords
Speeding up SDS-PAGE: theory and experiment.
Sign Up to like & getrecommendations! Published in 2023 at "Electrophoresis"
DOI: 10.1002/elps.202300011
Abstract: In order to accelerate SDS-PAGE, here we propose an optimized version of the technique enabled by experimental tuning reinforced by theoretical description. In the resulting system, the gel buffer was diluted two-fold and supplemented with… read more here.
Keywords: page theory; speeding sds; sds page; theory experiment ... See more keywords
SDS‐PAGE fractionation to increase metaproteomic insight into the taxonomic and functional composition of microbial communities for biogas plant samples
Sign Up to like & getrecommendations! Published in 2018 at "Engineering in Life Sciences"
DOI: 10.1002/elsc.201800062
Abstract: Metaproteomics represent an important tool for the taxonomic and functional investigation of microbial communities in humans, environment, and technical applications. Due to the high complexity of the microbial communities, protein, and peptide fractionation is applied… read more here.
Keywords: taxonomic functional; functional composition; composition microbial; sds page ... See more keywords
Dermatophagoides farinae allergen Der f 9: Cloning, expression, purification, characterization and IgE‐binding in children with atopic asthma
Sign Up to like & getrecommendations! Published in 2017 at "Pediatric Pulmonology"
DOI: 10.1002/ppul.23526
Abstract: Background: The house dust mite species Dermatophagoides farinae releases allergens that cause allergies and asthma worldwide. This study sought to clone and express the full‐length cDNA encoding the group 9 allergen of D. farinae (Der… read more here.
Keywords: full length; dermatophagoides farinae; expression; der ... See more keywords
Site-Specific Detection of Tyrosine Phosphorylated CD95 Following Protein Separation by Conventional and Phospho-Protein Affinity SDS-PAGE.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6780-3_16
Abstract: Phosphorylation of two tyrosines in the death domain of CD95 is a critical mechanism in determining the receptor's choices between cell death and survival signals. Recently, site-specific monoclonal antibodies against phosphorylated tyrosines of CD95 have… read more here.
Keywords: site specific; sds page; specific detection; cd95 ... See more keywords
The No-Nonsens SDS-PAGE.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7546-4_8
Abstract: The discontinuous polyacrylamide gel electrophoresis system devised by Laemmli (Nature 227:680-685, 1970) has not only been used in numerous laboratories but has also been modified in several ways since its birth. In our laboratories, we… read more here.
Keywords: protein; page; nonsens sds; biology ... See more keywords
Determination of Protein Molecular Weights on SDS-PAGE.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8793-1_10
Abstract: An apparent molecular weight (MW) of a protein can be determined from the migration distance of a protein complexed with a strong cationic detergent sodium dodecyl sulfate (SDS) separated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis… read more here.
Keywords: molecular weights; page; weights sds; protein molecular ... See more keywords
Utilization of SDS-PAGE and histochemistry for pharmacognostical studies on selected mangroves and halophytes from the Pichavaram, South India
Sign Up to like & getrecommendations! Published in 2019 at "Environment, Development and Sustainability"
DOI: 10.1007/s10668-019-00538-7
Abstract: This study focused on revealing the pharmacognostical marker, viz. histochemical and protein profiles for Avicennia marina (Forssk.) Vierh., Rhizophora mucronata Lam, Excoecaria agallocha L., Suaeda maritima Dumort and Sesuvium portulacastrum L. The proteins of the… read more here.
Keywords: page; selected mangroves; mangroves halophytes; page histochemistry ... See more keywords
Domain unfolding of monoclonal antibody fragments revealed by non-reducing SDS-PAGE
Sign Up to like & getrecommendations! Published in 2018 at "Biochemistry and Biophysics Reports"
DOI: 10.1016/j.bbrep.2018.10.004
Abstract: Monoclonal antibodies and derived fragments are used extensively both experimentally and therapeutically. Thorough characterization of such antibodies is necessary and includes assessment of their thermal and storage stabilities. Thus, assessment of the underlying conformational stabilities… read more here.
Keywords: reducing sds; non reducing; antibody; sds page ... See more keywords
Quality Monitoring of Porous Zein Scaffolds: A Novel Biomaterial
Sign Up to like & getrecommendations! Published in 2017 at "Engineering"
DOI: 10.1016/j.eng.2017.01.001
Abstract: Abstract Our previous studies have shown that zein has good biocompatibility and good mechanical properties. The first product from a porous scaffold of zein, a resorbable bone substitute, has passed the biological evaluation of medical… read more here.
Keywords: porous zein; quality monitoring; zein; sds page ... See more keywords