Evaluation of the efficiency and utility of recombinant enzyme-free seamless DNA cloning methods
Sign Up to like & getrecommendations! Published in 2017 at "Biochemistry and Biophysics Reports"
DOI: 10.1016/j.bbrep.2017.01.010
Abstract: Simple and low-cost recombinant enzyme-free seamless DNA cloning methods have recently become available. In vivo Escherichia coli cloning (iVEC) can directly transform a mixture of insert and vector DNA fragments into E. coli, which are… read more here.
Keywords: dna; cloning methods; seamless; seamless dna ... See more keywords
RedEx: a method for seamless DNA insertion and deletion in large multimodular polyketide synthase gene clusters
Sign Up to like & getrecommendations! Published in 2020 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaa956
Abstract: Abstract Biosynthesis reprograming is an important way to diversify chemical structures. The large repetitive DNA sequences existing in polyketide synthase genes make seamless DNA manipulation of the polyketide biosynthetic gene clusters extremely challenging. In this… read more here.
Keywords: dna; polyketide synthase; gene clusters; seamless dna ... See more keywords
Versatile seamless DNA vector production in E. coli using enhanced phage lambda integrase
Sign Up to like & getrecommendations! Published in 2022 at "PLoS ONE"
DOI: 10.1371/journal.pone.0270173
Abstract: Seamless DNA vectors derived from bacterial plasmids are devoid of bacterial genetic elements and represent attractive alternatives for biomedical applications including DNA vaccines. Larger scale production of seamless vectors employs engineered Escherichia coli strains in… read more here.
Keywords: dna; seamless dna; lambda integrase; production ... See more keywords