A bicistronic vector with destabilized mRNA secondary structure yields scalable higher titer expression of human neurturin in E. coli
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26299
Abstract: Human neurturin (NTN) is a cystine knot growth factor with potential therapeutic use in diseases such as Parkinson's and diabetes. Scalable high titer production of native NTN is particularly challenging because of the cystine knot… read more here.
Keywords: bicistronic vector; secondary structure; mrna secondary; expression ... See more keywords
A Conditionally Fluorescent Peptide Reporter of Secondary Structure Modulation
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DOI: 10.1002/cbic.201800377
Abstract: Proteins containing intrinsic disorder often form secondary structure upon interaction with a binding partner. Modulating such structures presents an approach for manipulating the resultant functional outcomes. Translational repressor protein 4E‐BP1 is an example of an… read more here.
Keywords: reporter; fluorescent peptide; conditionally fluorescent; secondary structure ... See more keywords
Probing the Secondary Structure of Individual Aβ40 Amorphous Aggregates and Fibrils by AFM‐IR Spectroscopy
Sign Up to like & getrecommendations! Published in 2020 at "ChemBioChem"
DOI: 10.1002/cbic.202000632
Abstract: Structural characterization of aggregates and fibrils of the Aβ protein is pivotal to the molecular‐level elucidation of Alzheimer's disease (AD). AFM‐IR spectroscopy provides nanoscale resolution, and thus allows the interrogation of individual aggregates and fibrils.… read more here.
Keywords: secondary structure; afm spectroscopy; amorphous aggregates; spectroscopy ... See more keywords
Amber Light Control of Peptide Secondary Structure by a Perfluoroaromatic Azobenzene Photoswitch
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DOI: 10.1002/cbic.202200570
Abstract: The incorporation of photoswitches into the molecular structure of peptides and proteins enables their dynamic photocontrol in complex biological systems. Here, a perfluorinated azobenzene derivative triggered by amber light was site‐specifically conjugated to cysteines in… read more here.
Keywords: structure; control peptide; secondary structure; peptide secondary ... See more keywords
The Impact of Secondary Structure on the Base‐Filling of N‐Methoxy‐1,3‐Oxazinane (MOANA) and N‐Methoxy‐1,3‐Oxazolidine Glycol Nucleic Acid (MOGNA) Oligonucleotides
Sign Up to like & getrecommendations! Published in 2024 at "Chembiochem"
DOI: 10.1002/cbic.202400666
Abstract: Various single‐stranded and hairpin‐forming DNA and 2'‐O‐methyl‐RNA oligonucleotides bearing a single (2R,3S)‐4‐(methoxyamino)butane‐1,2,3‐triol residue esterified from either O1 and O2 or O1 and O3 were synthesized. Incubation of these oligonucleotides with equimolar mixtures of formylmethyl derivatives… read more here.
Keywords: impact secondary; filling methoxy; structure base; secondary structure ... See more keywords
A chemometric study combined with spectroscopy for the quantification of secondary structure of flagellar‐associated protein 174 (FAP174)
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DOI: 10.1002/cem.3221
Abstract: The secondary structure analyses of proteins hold immense importance in the field of protein science because it plays a vital role in its hierarchical classification. It is the most important transitional step in the prediction… read more here.
Keywords: protein; secondary structure; fap174; spectroscopy ... See more keywords
Hemin and bile pigments are the secondary structure regulators of intrinsically disordered antimicrobial peptides.
Sign Up to like & getrecommendations! Published in 2018 at "Chirality"
DOI: 10.1002/chir.22784
Abstract: The interaction of protoporphyrin compounds of human origin with the major bee venom component melittin (26 a.a., Z +6) and its hybrid derivative (CM15, 15 a.a., Z +6) were studied by a combination of various… read more here.
Keywords: pigments secondary; secondary structure; hemin bile; intrinsically disordered ... See more keywords
Secondary structure analysis of peptides with relevance to iron–sulfur cluster nesting
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DOI: 10.1002/jcc.25741
Abstract: Peptides coordinated to iron–sulfur clusters, referred to as maquettes, represent a synthetic strategy for constructing biomimetic models of iron–sulfur metalloproteins. These maquettes have been successfully employed as building blocks of engineered heme‐containing proteins with electron‐transfer… read more here.
Keywords: iron; cluster nesting; secondary structure; iron sulfur ... See more keywords
Experimental Methods for Characterizing the Secondary Structure and Thermal Properties of Silk Proteins.
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DOI: 10.1002/marc.201800390
Abstract: Silk proteins are biopolymers produced by spinning organisms that have been studied extensively for applications in materials engineering, regenerative medicine, and devices due to their high tensile strength and extensibility. This remarkable combination of mechanical… read more here.
Keywords: methods characterizing; experimental methods; secondary structure; silk proteins ... See more keywords
A comprehensive guide for secondary structure and tertiary structure determination in peptides and proteins by circular dichroism spectrometer
Sign Up to like & getrecommendations! Published in 2024 at "Journal of Peptide Science"
DOI: 10.1002/psc.3648
Abstract: Secondary structure refers to highly regular local sub‐structures formed by the polypeptide backbone through hydrogen bonding. The two main types of secondary structures are α‐helices and β‐strands (which can form β‐sheets). The development of a… read more here.
Keywords: guide secondary; structure; circular dichroism; comprehensive guide ... See more keywords
RNA structure, binding, and coordination in Arabidopsis
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DOI: 10.1002/wrna.1426
Abstract: From the moment of transcription, up through degradation, each RNA transcript is bound by an ever‐changing cohort of RNA binding proteins. The binding of these proteins is regulated by both the primary RNA sequence, as… read more here.
Keywords: rna secondary; rna; secondary structure; rna structure ... See more keywords