Efficient secretory production of large‐size heterologous enzymes in Bacillus subtilis: A secretory partner and directed evolution
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27478
Abstract: Secretory production of recombinant proteins provides a simple approach to the production and purification of target proteins in the enzyme industry. We developed a combined strategy for the secretory production of three large‐size heterologous enzymes… read more here.
Keywords: large size; production; secretory; size heterologous ... See more keywords
Secretory production of N-glycan-deleted glycoprotein in Aspergillus oryzae.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of bioscience and bioengineering"
DOI: 10.1016/j.jbiosc.2019.12.006
Abstract: The pharmaceutical industry has a high demand for glycoprotein production. The glycoform of glycoproteins is crucial for pharmacological activity. However, in general, cells produce glycoproteins with a heterologous glycoform, which is unfavorable for making uniform,… read more here.
Keywords: secretory production; aspergillus oryzae; aoglycodelete strain; production glycan ... See more keywords
Secretory Production of Tocotrienols in Saccharomyces cerevisiae.
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DOI: 10.1021/acssynbio.1c00484
Abstract: Tocotrienols as important components of vitamin E have attracted increasing attention, with recent progress made in their heterologous biosynthesis, but all as intracellular products. Aiming to further improve the tocotrienol production capacity of engineered yeast… read more here.
Keywords: tocotrienols saccharomyces; production; secretory production; saccharomyces cerevisiae ... See more keywords
Stepwise modifications of genetic parts reinforce the secretory production of nattokinase in Bacillus subtilis
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DOI: 10.1111/1751-7915.13298
Abstract: Nattokinase (NK) is an important serine‐protease with direct fibrinolytic activity involving the prevention of cardiovascular disease as an antithrombotic agent. Dozens of studies have focused on the characterization of intrinsic novel promoters and signal peptides… read more here.
Keywords: nattokinase; production; secretory; bacillus subtilis ... See more keywords