High-Throughput Mutant Screening in Vibrio cholerae via Transposon Sequencing.
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DOI: 10.1101/pdb.prot108185
Abstract: Transposon mutagenesis greatly facilitates the study of gene function in microorganisms ranging from viruses to fungi. Traditionally, one would study individual transposon mutants with interesting phenotypes one mutant at a time. Here, we describe methods… read more here.
Keywords: section outlines; transposon sequencing; vibrio cholerae; transposon ... See more keywords
CRISPR Gene Editing of Virulent Bacteriophage ICP1.
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DOI: 10.1101/pdb.prot108316
Abstract: Tools for site-directed mutagenesis of virulent bacteriophages (phages; viruses of bacteria) have traditionally lagged those for bacteria, hindering their study. CRISPR gene editing represents a new and highly efficient method for editing virulent phage genomes.… read more here.
Keywords: gene editing; section outlines; crispr gene; icp1 ... See more keywords