Aggregation Profiling of C9orf72 Dipeptide Repeat Proteins Transgenically Expressed in Drosophila melanogaster Using an Analytical Ultracentrifuge Equipped with Fluorescence Detection.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9678-0_6
Abstract: The recent development of a fluorescence detection system for the analytical ultracentrifuge has allowed for the characterization of protein size and aggregation in complex mixtures. Protocols are described here to analyze protein aggregation seen in… read more here.
Keywords: analytical ultracentrifuge; sedimentation velocity; aggregation; fluorescence detection ... See more keywords
Using modern approaches to sedimentation velocity to detect conformational changes in proteins
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DOI: 10.1007/s00249-020-01453-w
Abstract: It has been known for decades that proteins undergo conformational changes in response to binding ligands. Such changes are usually accompanied by a loss of entropy by the protein, and thus conformational changes are integral… read more here.
Keywords: using modern; sedimentation velocity; modern approaches; conformational changes ... See more keywords
Sedimentation velocity analytical ultracentrifugation for characterization of therapeutic antibodies
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DOI: 10.1007/s12551-017-0374-3
Abstract: Sedimentation velocity analytical ultracentrifugation (SV-AUC) coupled with direct computational fitting of the observed concentration profiles (sedimentating boundary) have been developed and widely used for the characterization of macromolecules and nanoparticles in solution. In particular, size… read more here.
Keywords: therapeutic antibodies; velocity analytical; sedimentation velocity; analytical ultracentrifugation ... See more keywords
Efficient data acquisition with three-channel centerpieces in sedimentation velocity.
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DOI: 10.1016/j.ab.2019.113414
Abstract: Three-channel 3D printed centerpieces with two sample sectors next to a joint solvent reference sector were recently described as a strategy to double the throughput of sedimentation velocity analytical ultracentrifugation experiments [Anal. Chem. 91 (2019)… read more here.
Keywords: sedimentation velocity; data acquisition; three channel; acquisition ... See more keywords
Quantitation of soluble aggregates by sedimentation velocity analytical ultracentrifugation using an optical alignment system - aspects of method validation.
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DOI: 10.1016/j.ab.2020.113837
Abstract: Sedimentation velocity analytical ultracentrifugation (SV-AUC) is routinely used for quantitation of soluble aggregates as an orthogonal technique to size-exclusion chromatography (SEC). SV-AUC presents many advantages over the SEC, yet lower precision of aggregate quantitation by… read more here.
Keywords: velocity analytical; system; quantitation soluble; quantitation ... See more keywords