Increasing Cell Seeding Density Improves Elastin Expression and Mechanical Properties in Collagen Gel-Based Scaffolds Cellularized with Smooth Muscle Cells.
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology journal"
DOI: 10.1002/biot.201700768
Abstract: Vascular tissue engineering combines cells with scaffold materials in vitro aiming the development of physiologically relevant vascular models. For natural scaffolds such as collagen gels, where cells can be mixed with the material solution before… read more here.
Keywords: collagen gel; seeding density; density; cell seeding ... See more keywords
Cell seeding simulation on micropatterned islands shows cell density depends on area to perimeter ratio, not on island size or shape.
Sign Up to like & getrecommendations! Published in 2020 at "Acta biomaterialia"
DOI: 10.1016/j.actbio.2020.02.035
Abstract: Protein micropatterned substrates have been used to control cell size, shape, and cell-cell contacts, characteristics that influence a range of cell behaviors from early cell differentiation to late stages of maturation. Knowing the initial island… read more here.
Keywords: seeding density; density; cell seeding; island ... See more keywords
Seeding density is critical for optimizing scalable feeder-free natural killer cell expansion
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DOI: 10.1016/j.jcyt.2020.03.240
Abstract: Background & Aim Generating clinical doses of natural killer (NK) cells is an important challenge for Chimeric Antigen Receptor (CAR)-NK cell therapies. Current manufacturing methods to expand NK cells include irradiated feeder cells. However, these… read more here.
Keywords: cell expansion; expansion; seeding density; purity ... See more keywords
Optimizing the Seeding Density of Human Mononuclear Cells to Improve the Purity of Highly Proliferative Mesenchymal Stem Cells
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DOI: 10.3390/bioengineering10010102
Abstract: Mesenchymal stem cells (MSCs) hold considerable promise for regenerative medicine. Optimization of the seeding density of mononuclear cells (MNCs) improves the proliferative and differentiation potential of isolated MSCs. However, the underlying mechanism is unclear. We… read more here.
Keywords: seeding density; density; mononuclear cells; highly proliferative ... See more keywords