Selectable marker recycling in the nonconventional yeast Xanthophyllomyces dendrorhous by transient expression of Cre on a genetically unstable vector
Sign Up to like & getrecommendations! Published in 2018 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-018-9496-1
Abstract: Selectable marker recycling is a basic technique in bioengineering. However, this technique is usually unavailable in non-model microorganisms. In this study, we proposed a simple and efficient method for selectable marker recycling in the astaxanthin-synthesizing… read more here.
Keywords: vector; cre; yeast; selectable marker ... See more keywords
EYK1 encoding erythrulose kinase as a catabolic selectable marker for genome editing in the non-conventional yeast Yarrowia lipolytica.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2017.05.012
Abstract: We report here on EYK1, encoding erythrulose kinase, as an efficient catabolic selectable marker for genome editing in Y. lipolytica. Compared to auxotrophic markers, EYK1 increases the growth rate of transformants and allows improved efficiency… read more here.
Keywords: selectable marker; erythrulose kinase; catabolic selectable; encoding erythrulose ... See more keywords
Cloning of the ben gene and its functional identification in Cordyceps militaris
Sign Up to like & getrecommendations! Published in 2021 at "Scientia Horticulturae"
DOI: 10.1016/j.scienta.2021.109953
Abstract: Abstract Selectable marker genes are very important for the screening of target mutants. At least two selectable marker genes are usually required to identify the function of a gene by gene knockout, gene complementation, and… read more here.
Keywords: gene; method; ben gene; selectable marker ... See more keywords
The streptothricin acetyltransferase (sat) gene as a positive selectable marker for methanogenic archaea.
Sign Up to like & getrecommendations! Published in 2019 at "FEMS microbiology letters"
DOI: 10.1093/femsle/fnz216
Abstract: A repertoire of sophisticated genetic tools has significantly enhanced studies of Methanosarcina genera, yet the lack of multiple positive selectable markers has limited the types of genetic experiments that can be performed. In this study,… read more here.
Keywords: positive selectable; sat gene; streptothricin acetyltransferase; selectable marker ... See more keywords
Co-targeting strategy for precise, scarless gene editing with CRISPR/Cas9 and donor ssODNs in Chlamydomonas.
Sign Up to like & getrecommendations! Published in 2021 at "Plant physiology"
DOI: 10.1093/plphys/kiab418
Abstract: Programmable site-specific nucleases, such as the clustered regularly interspaced short palindromic repeat (CRISPR)/ CRISPR-associated protein 9 (Cas9) ribonucleoproteins (RNPs), have allowed creation of valuable knockout mutations and targeted gene modifications in Chlamydomonas (Chlamydomonas reinhardtii). However,… read more here.
Keywords: selectable marker; precise; precise scarless; ssodns ... See more keywords
ptxD/Phi as alternative selectable marker system for genetic transformation for bio-safety concerns: a review
Sign Up to like & getrecommendations! Published in 2021 at "PeerJ"
DOI: 10.7717/peerj.11809
Abstract: Antibiotic and herbicide resistance genes are the most common marker genes for plant transformation to improve crop yield and food quality. However, there is public concern about the use of resistance marker genes in food… read more here.
Keywords: marker genes; system; selection; transformation ... See more keywords