Construction and characterization of the GFAT gene as a novel selection marker in Aspergillus nidulans
Sign Up to like & getrecommendations! Published in 2018 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-018-9185-0
Abstract: Glutamine:fructose-6-phosphate aminotransferase (GFAT) catalyzes the formation of glucosamine-6-phosphate, and its gene is one of the genes essential for microbes. Using the GFAT-encoding gene can prevent the use of a drug-resistant gene as a selection marker… read more here.
Keywords: selection marker; gfat; gene; gfat gene ... See more keywords
SSA-mediated selection marker gene activation enhances relative gene targeting efficiency in plants
Sign Up to like & getrecommendations! Published in 2025 at "Horticulture Research"
DOI: 10.1093/hr/uhaf196
Abstract: Abstract The precise manipulation of genome sequences through gene targeting (GT) is beneficial; however, the low efficiency of homology-directed repair (HDR) in seed plants has made GT difficult to achieve. Generation of double-strand breaks (DSBs)… read more here.
Keywords: selection marker; seed; efficiency; gene targeting ... See more keywords
Template plasmids optimized for deletion of multiple genes in yeast Saccharomyces cerevisiae
Sign Up to like & getrecommendations! Published in 2024 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.01320-24
Abstract: ABSTRACT For Saccharomyces cerevisiae, gene knockout is routinely performed by transformation with a linear DNA cassette consisting of a selection marker gene flanked by upstream and downstream sequences homologous to a target gene. Over the… read more here.
Keywords: selection marker; selection; gene; multiple genes ... See more keywords
Protoplast-mediated transformation of Madurella mycetomatis using hygromycin resistance as a selection marker
Sign Up to like & getrecommendations! Published in 2024 at "PLOS Neglected Tropical Diseases"
DOI: 10.1371/journal.pntd.0012092
Abstract: Madurella mycetomatis is the main cause of mycetoma, a chronic granulomatous infection for which currently no adequate therapy is available. To improve therapy, more knowledge on a molecular level is required to understand how M.… read more here.
Keywords: selection marker; mycetomatis; mediated transformation; using hygromycin ... See more keywords
The Increase in Protein and Plasmid Yields of E. coli with Optimized Concentration of Ampicillin as Selection Marker.
Sign Up to like & getrecommendations! Published in 2017 at "Iranian journal of biotechnology"
DOI: 10.15171/ijb.1467
Abstract: Background:Escherichia coli is still the common host for ing and heterologous protein expression. Various strategies have been employed to increase protein expression in E. coli, but, it seems that external factors such as selection marker… read more here.
Keywords: protein; plasmid yields; protein plasmid; yields coli ... See more keywords
A selection marker-free method for gene deletion and editing in baculovirus genomes.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of virological methods"
DOI: 10.2139/ssrn.4154109
Abstract: Here, we develop a simple, efficient, bacmid-based, selection marker-free method for gene deletion and editing in baculovirus genomes. Specifically, based on pFastbac1, a donor plasmid with long left and right homology arms but without a… read more here.
Keywords: baculovirus; selection marker; free method; method ... See more keywords
PcMuORP1, an Oxathiapiprolin-Resistance Gene, Functions as a Novel Selection Marker for Phytophthora Transformation and CRISPR/Cas9 Mediated Genome Editing
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2019.02402
Abstract: Phytophthora, a genus of oomycetes, contains many devastating plant pathogens, which cause substantial economic losses worldwide. Recently, CRISPR/Cas9-based genome editing tool was introduced into Phytophthora to delineate the functionality of individual genes. The available selection… read more here.
Keywords: phytophthora transformation; selection marker; crispr cas9; selection ... See more keywords
Chili Pepper AN2 (CaAN2): A Visible Selection Marker for Nondestructive Monitoring of Transgenic Plants
Sign Up to like & getrecommendations! Published in 2022 at "Plants"
DOI: 10.3390/plants11060820
Abstract: Selecting transformed plants is generally time consuming and laborious. To develop a method for transgenic plant selection without the need for antibiotics or herbicides, we evaluated the suitability of the R2R3 MYB transcription factor gene… read more here.
Keywords: visible selection; caan2; chili pepper; tobacco ... See more keywords