Functional Analysis of Histidine‐Dependent Self‐Cleaving RNAs Obtained by In Vitro Selection
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DOI: 10.1002/cbic.202500411
Abstract: Ribozymes, ribonucleic acids with enzymatic functions, have not yet been practically used in nucleic acid medicine, possibly due to their low activity levels. We hypothesized that functional RNAs with high activity can be obtained by… read more here.
Keywords: histidine dependent; self cleaving; histidine; selection ... See more keywords
Circular RNAs Biogenesis in Eukaryotes Through Self-Cleaving Hammerhead Ribozymes.
Sign Up to like & getrecommendations! Published in 2018 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-981-13-1426-1_5
Abstract: Circular DNAs are frequent genomic molecules, especially among the simplest life beings, whereas circular RNAs have been regarded as weird nucleic acids in biology. Now we know that eukaryotes are able to express circRNAs, mostly… read more here.
Keywords: hammerhead ribozymes; circular rnas; biogenesis eukaryotes; self cleaving ... See more keywords
Application of RtcB ligase to monitor self-cleaving ribozyme activity by RNA-seq
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DOI: 10.1515/hsz-2021-0408
Abstract: Abstract Self-cleaving ribozymes are catalytic RNAs and can be found in all domains of life. They catalyze a site-specific cleavage that results in a 5′ fragment with a 2′,3′ cyclic phosphate (2′,3′ cP) and a… read more here.
Keywords: self cleaving; rtcb ligase; rna seq; ribozyme ... See more keywords
The Inducible Intein-Mediated Self-Cleaving Tag (IIST) System: A Novel Purification and Amidation System for Peptides and Proteins
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DOI: 10.3390/molecules26195948
Abstract: An efficient self-cleavable purification tag could be a powerful tool for purifying recombinant proteins and peptides without additional proteolytic processes using specific proteases. Thus, the intein-mediated self-cleavage tag was developed and has been commercially available… read more here.
Keywords: inducible intein; system; iist system; intein mediated ... See more keywords