Bioassay-based detection of Toxoplasma gondii in free-range chickens from Khorramabad, Iran: comparison of direct microscopy and semi-nested PCR
Sign Up to like & getrecommendations! Published in 2020 at "Molecular Biology Reports"
DOI: 10.1007/s11033-020-05539-8
Abstract: This study aimed to investigate the occurrence of anti-Toxoplasma gondii antibodies in free-range chickens from Khorramabad, western Iran, and also to compare the performance of direct microscopy and semi-nested PCR in mice bioassayed with tissues… read more here.
Keywords: direct microscopy; semi nested; free range; microscopy ... See more keywords
A highly sensitive semi-nested real-time PCR utilizing oligospermine-conjugated degenerate primers for the detection of diverse strains of small ruminant lentiviruses.
Sign Up to like & getrecommendations! Published in 2020 at "Molecular and cellular probes"
DOI: 10.1016/j.mcp.2020.101528
Abstract: Small ruminant lentiviruses (SRLVs) are highly diverse retroviruses infecting sheep and goats. Although PCR-based testing is being utilized for diagnostics, its application is hampered by various factors. These include, among others, the exceptionally high genetic… read more here.
Keywords: semi nested; semi; dna; highly sensitive ... See more keywords
Development of RT-qPCR and semi-nested RT-PCR assays for molecular diagnosis of hantavirus pulmonary syndrome
Sign Up to like & getrecommendations! Published in 2019 at "PLoS Neglected Tropical Diseases"
DOI: 10.1371/journal.pntd.0007884
Abstract: Hantavirus Pulmonary Syndrome is an, often fatal, emerging zoonotic disease in the Americas caused by hantaviruses (family: Hantaviridae). In Brazil, hantavirus routine diagnosis is based on serology (IgM-ELISA) while RT-PCR is often used to confirm… read more here.
Keywords: semi nested; hantavirus pulmonary; nested pcr;
A comparison of the efficiency of molecular and conventional methods in the diagnosis of cutaneous leishmaniosis.
Sign Up to like & getrecommendations! Published in 2021 at "Annals of parasitology"
DOI: 10.17420/ap6701.310
Abstract: Leishmaniosis is one of the most important vector borne diseases. Among different forms of the disease, cutaneous leishmaniosis (CL) is the most common. Determining the method of definitive diagnosis for the disease has been the… read more here.
Keywords: semi nested; microscopy; cutaneous leishmaniosis; leishmaniosis ... See more keywords
[Application of two RT-PCR methods for detection of norovirus in market-sold oysters and norovirus genetic characteristic analysis, a survey conducted in Beijing].
Sign Up to like & getrecommendations! Published in 2022 at "Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi"
DOI: 10.3760/cma.j.cn112338-20210519-00411
Abstract: Objective: To evaluate the application of real-time RT-PCR and semi-nested RT-PCR in the detection of norovirus in oysters and analyzing the genetic characteristics of the isolates. Methods: Real-time fluorescent RT-PCR and semi-nested RT-PCR were used… read more here.
Keywords: semi nested; two pcr; detection; pcr ... See more keywords