Comparison of the separation of proteins of wide-ranging molecular weight via trilobal polypropylene capillary-channeled polymer fiber, commercial superficiously porous, and commercial size exclusion columns.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of separation science"
DOI: 10.1002/jssc.202100891
Abstract: Reversed phase and size-exclusion chromatography methods are commonly used for protein separations, though based on distinctly different principles. Reversed phase methods yield hydrophobicity-based (loosely-termed) separation of proteins on porous supports, but tend to be limited… read more here.
Keywords: separation; separation proteins; size exclusion; fiber ... See more keywords
CRISPR-assisted transcription activation by phase separation proteins.
Sign Up to like & getrecommendations! Published in 2023 at "Protein & cell"
DOI: 10.1093/procel/pwad013
Abstract: The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system has been widely used for genome engineering and transcriptional regulation in many different organisms. Current CRISPR-activation (CRISPRa) platforms often require multiple components because of inefficient transcriptional… read more here.
Keywords: dcas9 vpr; phase separation; separation proteins; activation ... See more keywords