KIF2C affects sperm cell differentiation in patients with Klinefelter syndrome, as revealed by RNA‐Seq and scRNA‐Seq data
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.13446
Abstract: Klinefelter syndrome (KS) is a leading contributor to male infertility and is characterised by complex and diverse clinical features; however, genetic changes in the KS transcriptome remain largely unknown. We therefore used transcriptomic and single‐cell… read more here.
Keywords: cell; scrna seq; seq; klinefelter syndrome ... See more keywords
Enabling evolutionary studies at multiple scales in Apocynaceae through Hyb‐Seq
Sign Up to like & getrecommendations! Published in 2020 at "Applications in Plant Sciences"
DOI: 10.1002/aps3.11400
Abstract: Premise Apocynaceae is the 10th largest flowering plant family and a focus for study of plant–insect interactions, especially as mediated by secondary metabolites. However, it has few genomic resources relative to its size. Target capture… read more here.
Keywords: seq; studies multiple; evolutionary studies; enabling evolutionary ... See more keywords
Evaluation of two public genome references for chinese hamster ovary cells in the context of rna‐seq based gene expression analysis
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26290
Abstract: RNA‐Seq is a powerful transcriptomics tool for mammalian cell culture process development. Successful RNA‐Seq data analysis requires a high quality reference for read mapping and gene expression quantification. Currently, there are two public genome references… read more here.
Keywords: seq; analysis; genome; gene ... See more keywords
SLAM‐seq reveals early transcriptomic response mechanisms upon glutamine deprivation in Chinese hamster ovary cells
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28320
Abstract: Mammalian cells frequently encounter subtle perturbations during recombinant protein production. Identifying the genetic factors that govern the cellular stress response can facilitate targeted genetic engineering to obtain production cell lines that demonstrate a higher stress… read more here.
Keywords: chinese hamster; seq; hamster ovary; slam seq ... See more keywords
RNA‐seq and ATAC‐seq analyses of multilineage differentiating stress enduring cells: Comparison with dermal fibroblasts
Sign Up to like & getrecommendations! Published in 2022 at "Cell Biology International"
DOI: 10.1002/cbin.11834
Abstract: The aim of this study is to characterize the molecular properties of multilineage differentiating stress‐enduring (Muse) cells compared with dermal fibroblasts (FBs) and to characterize differences in their transcriptomes and open chromatin regions that are… read more here.
Keywords: muse cells; atac seq; seq; seq analyses ... See more keywords
Big Data: The good, the bad and the ugly
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Cancer"
DOI: 10.1002/ijc.33466
Abstract: Although we are reflecting about machine ethics, learn how to live with machines and realize how easy it is to fool deep neural networks, we better do not forget about the more mundane problems associated… read more here.
Keywords: seq; data sets; quality; big data ... See more keywords
Integrated mRNA‐Seq and miRNA‐Seq analysis of PLCγ2‐overexpressing hepatocarcinoma cells and identification of the associated miRNA‐mRNA network
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Cellular Biochemistry"
DOI: 10.1002/jcb.29294
Abstract: Our previous study has discovered the positive effect of phospholipase Cγ 2 (PLCγ2) on the growth of hepatocarcinoma cells; however, the underlying mechanism is far from being understood. For this reason, this study attempts to… read more here.
Keywords: seq; mirna; hepatocarcinoma cells; mirna mrna ... See more keywords
Sequential application of copy number variation sequencing and quantitative fluorescence polymerase chain reaction in genetic analysis of miscarriage and stillbirth.
Sign Up to like & getrecommendations! Published in 2023 at "Molecular genetics & genomic medicine"
DOI: 10.1002/mgg3.2187
Abstract: BACKGROUND Copy number variation sequencing (CNV-seq) could detect most chromosomal abnormalities except polyploidy, and quantitative fluorescence polymerase chain reaction (QF-PCR) is a supplementary method to CNV-seq in triploid detection. This study aimed to evaluate the… read more here.
Keywords: chromosomal abnormalities; cnv seq; seq; analysis ... See more keywords
Well‐Paired‐Seq: A Size‐Exclusion and Locally Quasi‐Static Hydrodynamic Microwell Chip for Single‐Cell RNA‐Seq
Sign Up to like & getrecommendations! Published in 2022 at "Small Methods"
DOI: 10.1002/smtd.202200341
Abstract: Single‐cell RNA sequencing (scRNA‐seq) is a powerful technology for revealing the heterogeneity of cellular states. However, existing scRNA‐seq platforms that utilize bead‐based technologies suffer from a large number of empty microreactors and a low cell/bead… read more here.
Keywords: well paired; cell; paired seq; seq ... See more keywords
Dynamic transcriptomic m5C and its regulatory role in RNA processing
Sign Up to like & getrecommendations! Published in 2021 at "Wiley Interdisciplinary Reviews: RNA"
DOI: 10.1002/wrna.1639
Abstract: RNA 5‐methylcytosine (m5C) is a prevalent RNA modification in multiple RNA species, including messenger RNAs (mRNAs), transfer RNAs (tRNAs), ribosomal RNAs (rRNAs), and noncoding RNAs (ncRNAs), and broadly distributed from archaea, prokaryotes to eukaryotes. The… read more here.
Keywords: seq; transcriptomic m5c; dynamic transcriptomic; rna ... See more keywords
Epitope Tagging ChIP-Seq of DNA Binding Proteins Using CETCh-Seq.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0301-7_1
Abstract: Chromatin immunoprecipitation followed by next-generation DNA sequencing (ChIP-seq) has been used to identify transcription factor (TF) binding proteins throughout the genome. Unfortunately, this approach traditionally requires commercially available, ChIP-seq grade antibodies that frequently fail to… read more here.
Keywords: seq; chip seq; binding proteins; cetch seq ... See more keywords