Complementary Methods for de novo Monoclonal Antibody Sequencing to Achieve Complete Sequence Coverage.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.0c00223
Abstract: Mass spectrometry is a powerful tool for de novo sequencing of novel proteins. Recent efforts in this area have mainly focused on liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Here we present an alternative… read more here.
Keywords: mass spectrometry; antibody; complementary methods; coverage ... See more keywords
Data-Independent Acquisition Protease-Multiplexing Enables Increased Proteome Sequence Coverage Across Multiple Fragmentation Modes.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.1c00960
Abstract: The use of multiple proteases has been shown to increase protein sequence coverage in proteomics experiments; however, due to the additional analysis time required, it has not been widely adopted in routine data-dependent acquisition (DDA)… read more here.
Keywords: sequence coverage; independent acquisition; data independent; sequence ... See more keywords
Validating Amino Acid Variants in Proteogenomics Using Sequence Coverage by Multiple Reads.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.2c00033
Abstract: Mass spectrometry-based proteome analysis implies matching the mass spectra of proteolytic peptides to amino acid sequences predicted from genomic sequences. Reliability of peptide variant identification in proteogenomic studies is often lacking. We propose a way… read more here.
Keywords: sequence coverage; coverage multiple; amino acid; multiple reads ... See more keywords
Expanding Proteome Coverage with CHarge Ordered Parallel Ion aNalysis (CHOPIN) Combined with Broad Specificity Proteolysis
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Proteome Research"
DOI: 10.1021/acs.jproteome.6b00915
Abstract: The “deep” proteome has been accessible by mass spectrometry for some time. However, the number of proteins identified in cells of the same type has plateaued at ∼8000–10 000 without ID transfer from reference proteomes/data. Moreover,… read more here.
Keywords: analysis; chopin; coverage; parallel ion ... See more keywords
Improving the Center Section Sequence Coverage of Large Proteins Using Stepped-Fragment Ion Protection Ultraviolet Photodissociation.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.1c00296
Abstract: Ultraviolet photodissociation (UVPD) mass spectrometry has gained attention in recent years for its ability to provide high sequence coverage of intact proteins. However, secondary dissociation of fragment ions, in which fragment ions subjected to multiple… read more here.
Keywords: sequence coverage; fragment ion; fragment ions; sequence ... See more keywords
Isotope Depletion Mass Spectrometry (ID-MS) for accurate mass determination and improved top-down sequence coverage of intact proteins.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.9b00119
Abstract: Top-down mass spectrometry (MS) is an increasingly important technique for protein characterization. However, in many biological MS experiments, the practicality of applying top-down methodologies is still limited at higher molecular mass. In large part this… read more here.
Keywords: depletion; sequence coverage; mass; mass spectrometry ... See more keywords
Preprocessing Sequence Coverage Data for More Precise Detection of Copy Number Variations
Sign Up to like & getrecommendations! Published in 2020 at "IEEE/ACM Transactions on Computational Biology and Bioinformatics"
DOI: 10.1109/tcbb.2018.2869738
Abstract: Copy number variation (CNV) is a type of genomic/genetic variation that plays an important role in phenotypic diversity, evolution, and disease susceptibility. Next generation sequencing (NGS) technologies have created an opportunity for more accurate detection… read more here.
Keywords: sequence coverage; copy number; detection; precise detection ... See more keywords
Optimization and Identification of Single Mutation in Hemoglobin Variants with 2,2,2 Trifluoroethanol Modified Digestion Method and Nano−LC Coupled MALDI MS/MS
Sign Up to like & getrecommendations! Published in 2022 at "Molecules"
DOI: 10.3390/molecules27196357
Abstract: Background: Hemoglobin (Hb) variants arise due to point mutations in globin chains and their pathological treatments rely heavily on the identification of the nature and location of the mutation in the globin chains. Traditional methods… read more here.
Keywords: sequence coverage; globin chains; sequence; method ... See more keywords